The biological significance of phospholipase C beta 1 gene mutation in mouse sperm in the acrosome reaction, fertilization, and embryo development

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Purpose: We carried out this study to evaluate the biological significance of phospholipase C BI gene mutation in mouse sperm in the acrosome reaction, fertilization, and embryo development. Methods: Study subjects were divided into two groups according to the sperm [intact phospholipase C (PLC) beta1 and PLC beta1(-/-) C57BL/6J x CBA F-1 mouse sperm] used. The positive acrosome reaction rate labeled with fluorescein isothiocyanate-Pisum sativum agglutinin, the fertilization rate, and the rate of embryos developed to the stage of morula or blastocyst in the two groups were compared. Results: The mouse sperm null for the PLC BI gene showed a lower acrosome reaction rate than control sperm (69.2 vs 50.9%, P < 0.05). And the fertilization rate and the rate of embryos developed to the stage of morula or blastocyst were also lower in the group using PLC beta1(-/-) mouse sperm compared to the intact group (P < 0.05; 73.5 vs 51.8% and 15.7 vs 4.3%, respectively). Conclusions: Mutation of the PLC beta1 gene in the mouse sperm reduces the acrosome reaction rate, fertilization rate, and embryo development rate, which may be the etiologic factors responsible for the low reproductive rate of PLC beta1(-/-) mouse.
Publisher
KLUWER ACADEMIC/PLENUM PUBL
Issue Date
2001-05
Language
English
Article Type
Article; Proceedings Paper
Citation

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, v.18, no.5, pp.305 - 310

ISSN
1058-0468
DOI
10.1023/A:1016622519228
URI
http://hdl.handle.net/10203/20734
Appears in Collection
BS-Journal Papers(저널논문)
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