DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sohn, Seung-Bum | ko |
dc.contributor.author | Kim, Tae-Yong | ko |
dc.contributor.author | Park, Jong-Myoung | ko |
dc.contributor.author | Lee, Sang-Yup | ko |
dc.date.accessioned | 2010-11-30T08:36:45Z | - |
dc.date.available | 2010-11-30T08:36:45Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2010-07 | - |
dc.identifier.citation | BIOTECHNOLOGY JOURNAL, v.5, no.7, pp.739 - 750 | - |
dc.identifier.issn | 1860-6768 | - |
dc.identifier.uri | http://hdl.handle.net/10203/20547 | - |
dc.description.abstract | Genome-scale metabolic models have been appearing with increasing frequency and have been employed in a wide range of biotechnological applications as well as in biological studies. With the metabolic model as a platform, engineering strategies have become more systematic and focused, unlike the random shotgun approach used in the past. Here we present the genome-scale metabolic model of the versatile Gram-negative bacterium Pseudomonas putida, which has gained widespread interest for various biotechnological applications. With the construction of the genome-scale metabolic model of P. putida KT2440, PpuMBEL1071, we investigated various characteristics of P. putida, such as its capacity for synthesizing polyhydroxyalkanoates (PHA) and degrading aromatics. Although P. putida has been characterized as a strict aerobic bacterium, the physiological characteristics required to achieve anaerobic survival were investigated. Through analysis of PpuMBEL1071, extended survival of P. putida under anaerobic stress was achieved by introducing the ackA gene from Pseudomonas aeruginosa and Escherichia coli. | - |
dc.description.sponsorship | The authors would like to thank Yu Kyung Jung for the strains used in the anaerobic experiments and Hyun Uk Kim for his aid in editing this manuscript. This work was supported by the Korean Systems Biology Research Project (20100002164) and WCU (World Class University) program (R322009000101420) of the Ministry of Education, Science and Technology (MEST) through the National Research Foundation of Korea. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | WILEY-V C H VERLAG GMBH | - |
dc.title | In silico genome-scale metabolic analysis of Pseudomonas putida KT2440 for polyhydroxyalkanoate synthesis, degradation of aromatics and anaerobic survival | - |
dc.type | Article | - |
dc.identifier.wosid | 000280622500010 | - |
dc.identifier.scopusid | 2-s2.0-77954714441 | - |
dc.type.rims | ART | - |
dc.citation.volume | 5 | - |
dc.citation.issue | 7 | - |
dc.citation.beginningpage | 739 | - |
dc.citation.endingpage | 750 | - |
dc.citation.publicationname | BIOTECHNOLOGY JOURNAL | - |
dc.identifier.doi | 10.1002/biot.201000124 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Lee, Sang-Yup | - |
dc.contributor.nonIdAuthor | Kim, Tae-Yong | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Anaerobic survival | - |
dc.subject.keywordAuthor | Genome-scale metabolic model | - |
dc.subject.keywordAuthor | Metabolic engineering | - |
dc.subject.keywordAuthor | PHA | - |
dc.subject.keywordAuthor | Systems biology | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | BACTERIAL POLYHYDROXYALKANOATES | - |
dc.subject.keywordPlus | CATABOLIC PATHWAYS | - |
dc.subject.keywordPlus | SYSTEMS BIOLOGY | - |
dc.subject.keywordPlus | DATABASE | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | AERUGINOSA | - |
dc.subject.keywordPlus | RECONSTRUCTION | - |
dc.subject.keywordPlus | BIOSYNTHESIS | - |
dc.subject.keywordPlus | RESOURCE | - |
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