Identification of novel sRNAs involved in biofilm formation, motility, and fimbriae formation in Escherichia coli

Cited 34 time in webofscience Cited 0 time in scopus
  • Hit : 438
  • Download : 302
Bacterial small RNAs (sRNAs) are known regulators in many physiological processes. In Escherichia coli, a large number of sRNAs have been predicted, among which only about a hundred are experimentally validated. Despite considerable research, the majority of their functions remain uncovered. Therefore, collective analysis of the roles of sRNAs in specific cellular processes may provide an effective approach to identify their functions. Here, we constructed a collection of plasmids overexpressing 99 individual sRNAs, and analyzed their effects on biofilm formation and related phenotypes. Thirty-three sRNAs significantly affecting these cellular processes were identified. No consistent correlations were observed, except that all five sRNAs suppressing type I fimbriae inhibited biofilm formation. Interestingly, IS118, yet to be characterized, suppressed all the processes. Our data not only reveal potentially critical functions of individual sRNAs in biofilm formation and other phenotypes but also highlight the unexpected complexity of sRNA-mediated metabolic pathways leading to these processes.
Publisher
NATURE PUBLISHING GROUP
Issue Date
2015-10
Language
English
Article Type
Article
Keywords

SMALL NONCODING RNAS; SMALL REGULATORY RNAS; GENE-EXPRESSION; E. COLI; TRANSCRIPTIONAL ACTIVATOR; COMPARATIVE GENOMICS; BACTERIAL PATHOGENS; CURLI SYNTHESIS; OUTER-MEMBRANE; LIFE-STYLE

Citation

SCIENTIFIC REPORTS, v.5

ISSN
2045-2322
DOI
10.1038/srep15287
URI
http://hdl.handle.net/10203/205256
Appears in Collection
CH-Journal Papers(저널논문)
Files in This Item
000362809500001.pdf(3.62 MB)Download
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 34 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0