Label-free aptasensor for platelet-derived growth factor (PDGF) protein

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A label-free aptasensor for platelet-derived growth factor (PDGF) protein is reported. The aptasensor uses mixed self-assembled monolayers (SAMs) composed of a thiol-modified PDGF binding aptamer and 6-mercaptohexanol (MCH) on a gold electrode. The SAMs were characterized by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and differential pulse voltammetry (DPV) before and after binding of the protein using [Fe(CN)(6)](3-/4-), a redox marker ion as an indicator for the formation of a protein-aptamer complex. The CVs at the PDGF modified electrode showed significant differences, such as changes in the peak currents and peak-to-peak separation, before and after binding of the target protein. The EIS spectra, in the form of Nyquist plots, were analyzed with a Randles circuit while the electron transfer resistance R-ct was used to monitor the binding of the target protein. The results showed that, without any modification to the aptamer, the target protein can be recognized effectively at the PDGF binding aptamer SAMs at the electrode surface. Control experiments using non-binding oligonucleotides assembled at the electrode surfaces also confirmed the results and showed that there was no formation of an aptamer-protein complex. The DPV signal at the aptamer functionalized electrode showed a linearly decreased marker ion peak current in a protein concentrations range of 1-40 nM. Thus, label-free detection of PDGF protein at an aptamer modified electrode has been demonstrated. (C) 2008 Elsevier B.V. All rights reserved.
Publisher
ELSEVIER SCIENCE BV
Issue Date
2008-04
Language
English
Article Type
Article
Keywords

APTAMER-BASED SENSORS; ELECTROCHEMICAL DETECTION; IMPEDANCE SPECTROSCOPY; GOLD NANOPARTICLES; BIOLOGICAL-FLUIDS; DNA; AMPLIFICATION; COMPLEX; SWITCH; TRANSDUCTION

Citation

ANALYTICA CHIMICA ACTA, v.613, no.2, pp.163 - 168

ISSN
0003-2670
DOI
10.1016/j.aca.2008.03.010
URI
http://hdl.handle.net/10203/20081
Appears in Collection
CH-Journal Papers(저널논문)
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