Green fluorescent protein nanopolygons as monodisperse supramolecular assemblies of functional proteins with defined valency

Cited 49 time in webofscience Cited 0 time in scopus
  • Hit : 730
  • Download : 642
Supramolecular protein assemblies offer novel nanoscale architectures with molecular precision and unparalleled functional diversity. A key challenge, however, is to create precise nano-assemblies of functional proteins with both defined structures and a controlled number of protein-building blocks. Here we report a series of supramolecular green fluorescent protein oligomers that are assembled in precise polygonal geometries and prepared in a monodisperse population. Green fluorescent protein is engineered to be self-assembled in cells into oligomeric assemblies that are natively separated in a single-protein resolution by surface charge manipulation, affording monodisperse protein (nano)polygons from dimer to decamer. Several functional proteins are multivalently displayed on the oligomers with controlled orientations. Spatial arrangements of protein oligomers and displayed functional proteins are directly visualized by a transmission electron microscope. By employing our functional protein assemblies, we provide experimental insight into multivalent protein-protein interactions and tools to manipulate receptor clustering on live cell surfaces.
Publisher
NATURE PUBLISHING GROUP
Issue Date
2015-05
Language
English
Article Type
Article
Citation

NATURE COMMUNICATIONS, v.6

ISSN
2041-1723
DOI
10.1038/ncomms8134
URI
http://hdl.handle.net/10203/199629
Appears in Collection
MSE-Journal Papers(저널논문)CH-Journal Papers(저널논문)
Files in This Item
000355533300004.pdf(3.32 MB)Download
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 49 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0