Reconstruction and characterization of stacked 3D tissue using a hepatic cell-patterned hydrogel paper = 간세포가 패턴된 수화젤 종이를 이용한 적층된 삼차원 조직의 구현 및 특성평가

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We fabricated liver lobule-like stacked hydrogel papers embedded hepatic cells for in vitro liver tissue reconstruction with negligible diffusion limitation. HepG2 cell-embedded mi-cropatterned hydrogel papers which had the width of 8000 μm, the length of 8660 μm and the height of 80-140 μm. The stacked hydrogel papers were fabricated via laminating and aligning the hepatic hydrogel papers in a PDMS jig. For drainage, the jig had many pillars of 170 μm in height. The cells could be cultured in the stacked hydrogel papers which had the height of 300-600 μm exceeding diffusion limit thickness (150-200 μm). The patterned structure with many openings facilitated cell-to-cell interaction and overcame the diffusion limitation, so it gave good viability more than 90% for much remaining HepG2 cells with compared to no pattern. Also, cell-to-cell interaction between each hydrogel paper led to the twofold increase of albumin secretion and the maintenance of urea secretion per million cells with compared to a hydrogel paper. Therefore, we suggest that the stacked hydrogel papers can be macroscale hepatic pseudo-tissues with complex microenvironments, over-coming diffusion limitation for biological study of liver.
Advisors
Park, Je-Kyunresearcher박제균
Description
한국과학기술원 : 바이오및뇌공학과,
Publisher
한국과학기술원
Issue Date
2014
Identifier
568889/325007  / 020123354
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 바이오및뇌공학과, 2014.2, [ ix, 35 p. ]

Keywords

Scale-up; Diffusion limitation; 간 조직공학; 대형화; Liver tissue engineering; 확산 제약

URI
http://hdl.handle.net/10203/196321
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=568889&flag=dissertation
Appears in Collection
BiS-Theses_Master(석사논문)
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