DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Chang, Yong-Keun | - |
dc.contributor.advisor | 장용근 | - |
dc.contributor.author | Huh, In-Young | - |
dc.contributor.author | 허인영 | - |
dc.date.accessioned | 2015-04-23T02:09:25Z | - |
dc.date.available | 2015-04-23T02:09:25Z | - |
dc.date.issued | 2014 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=568945&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/196276 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생명화학공학과, 2014.2, [ v, 50 p. ] | - |
dc.description.abstract | An experimental and modelling study has been done for the hydrolysis of agarose to produce ne-oagarobiose by using a mixture of two different ß-agarases: AgaG1 screened from Alteromonas sp. GNUM1; and DagB from Streptomyces coelicolor A3(2). Experiments based on the central compo-site design (CCD) were carried out for various combinations of the amounts (or activity levels) of these two enzymes in the solution. Based on the experimental data, quadratic models or response surfaces quantitatively describing the effects of both enzymes on the volumetric productivity have been developed. The reference activity levels of AgaG1 and DagB for the CCD were identified when the reaction time was 12 hrs. In the case of DagB, 0.1 U/mL was enough to completely hy-drolysis of 10 g/L agarose in 12 hours, and in case of AgaG1, 0.7 U/mL was enough. The first-round CCD experiments with these reference points gave a model equation of y = 0.69 + 0.17A + 0.081B - 0.047A2 + 0.057AB, where y, A, and B denoted the productivity, the activity of AgaG1, and the activity of DagB, respectively. Its validity was confirmed by ANOVA. The second-round CCD experiments based on 6 hours of reaction gave a model of y = 0.93 + 0.26A + 0.157B + 0.14AB. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | agarose hydrolysis | - |
dc.subject | 중심합성법 | - |
dc.subject | 생산성 모델링 | - |
dc.subject | 베타아가로오스 | - |
dc.subject | 네오아가로바이오스 | - |
dc.subject | 아가로오스 분해 | - |
dc.subject | neoagarobiose | - |
dc.subject | ß-agarose | - |
dc.subject | productivity modeling | - |
dc.subject | CCD | - |
dc.title | Modeling of neoagarobiose production by enzymatic hydrolysis of agarose | - |
dc.title.alternative | 아가로오즈의 효소 분해에 의한 네오아가로바이오스 생산 모델 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 568945/325007 | - |
dc.description.department | 한국과학기술원 : 생명화학공학과, | - |
dc.identifier.uid | 020123767 | - |
dc.contributor.localauthor | Chang, Yong-Keun | - |
dc.contributor.localauthor | 장용근 | - |
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