A penicillamine biosensor based on tyrosinase immobilized on nano-Au/PAMAM dendrimer modified gold electrode

Cited 17 time in webofscience Cited 0 time in scopus
  • Hit : 191
  • Download : 427
Gold electrodes were modified with submonolayers of 3-mercaptopropionic acid and further reacted with poly(amidoamine) (PAMAM) dendrimers to obtain thin films. The high affinity of PAMAM dendrimer for nano-Au with its amine groups was used to realize the role of nano-Au as an intermediator to immobilize the enzyme of tyrosinase. The characterization of the modified electrode was investigated by cyclic voltammetry, electrochemical impedance spectroscopy and atomic force microscopy (AFM). Tyrosinase can catalyze the oxidation of catechol to o-benzoquinone. When penicillamine was added to the solution, it reacted with o-benzoquinone to form the corresponding thioquinone derivatives, which resulted in decrease of the reduction current of o-benzoquinone. Based on this, a new electrochemical sensor for determination of penicillamine has been developed.
Publisher
WILEY-V C H VERLAG GMBH
Issue Date
2007-12
Language
English
Article Type
Article
Keywords

ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY; FERROCENYL-TETHERED DENDRIMER; SPECTROPHOTOMETRIC DETERMINATION; LIQUID-CHROMATOGRAPHY; RHEUMATOID-ARTHRITIS; DOSAGE FORMS; DERIVATIZATION; THIOLS; COMPLEXATION; OXIDATION

Citation

ELECTROANALYSIS, v.19, no.23, pp.2428 - 2436

ISSN
1040-0397
DOI
10.1002/elan.200703968
URI
http://hdl.handle.net/10203/19328
Appears in Collection
CH-Journal Papers(저널논문)
Files in This Item
000251665800005.pdf(835.87 kB)Download
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 17 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0