Kinesin-II recruits Armadillo and Dishevelled for Wingless signaling in Drosophila

Cited 9 time in webofscience Cited 0 time in scopus
  • Hit : 316
  • Download : 0
DC FieldValueLanguage
dc.contributor.authorLinh Thuong Vuongko
dc.contributor.authorMukhopadhyay, Bibhashko
dc.contributor.authorChoi, Kwang-Wookko
dc.date.accessioned2014-12-16-
dc.date.available2014-12-16-
dc.date.created2014-09-30-
dc.date.created2014-09-30-
dc.date.issued2014-08-
dc.identifier.citationDEVELOPMENT, v.141, no.16, pp.3222 - 3232-
dc.identifier.issn0950-1991-
dc.identifier.urihttp://hdl.handle.net/10203/192608-
dc.description.abstractWingless (Wg)/Wnt signaling is fundamental in metazoan development. Armadillo (Arm)/beta-catenin and Dishevelled (Dsh) are key components of Wnt signal transduction. Recent studies suggest that intracellular trafficking of Wnt signaling components is important, but underlying mechanisms are not well known. Here, we show that Klp64D, the Drosophila homolog of Kif3A kinesin II subunit, is required for Wg signaling by regulating Arm during wing development. Mutations in klp64D or RNAi cause wing notching and loss of Wg target gene expression. The wing notching phenotype by Klp64D knockdown is suppressed by activated Arm but not by Dsh, suggesting that Klp64D is required for Arm function. Furthermore, klp64D and arm mutants show synergistic genetic interaction. Consistent with this genetic interaction, Klp64D directly binds to the Arm repeat domain of Arm and can recruit Dsh in the presence of Arm. Overexpression of Klp64D mutated in the motor domain causes dominant wing notching, indicating the importance of the motor activity. Klp64D shows subcellular localization to intracellular vesicles overlapping with Arm and Dsh. In klp64D mutants, Arm is abnormally accumulated in vesicular structures including Golgi, suggesting that intracellular trafficking of Arm is affected. Human KIF3A can also bind beta-catenin and rescue klp64D RNAi phenotypes. Taken together, we propose that Klp64D is essential for Wg signaling by trafficking of Arm via the formation of a conserved complex with Arm.-
dc.languageEnglish-
dc.publisherCOMPANY OF BIOLOGISTS LTD-
dc.subjectBETA-CATENIN-
dc.subjectCHOLINE-ACETYLTRANSFERASE-
dc.subjectINTRACELLULAR-TRANSPORT-
dc.subjectEMBRYONIC EPIDERMIS-
dc.subjectCUT EXPRESSION-
dc.subjectCELL-ADHESION-
dc.subjectIN-VIVO-
dc.subjectGENE-
dc.subjectPROTEIN-
dc.subjectNOTCH-
dc.titleKinesin-II recruits Armadillo and Dishevelled for Wingless signaling in Drosophila-
dc.typeArticle-
dc.identifier.wosid000341305200014-
dc.identifier.scopusid2-s2.0-84905454817-
dc.type.rimsART-
dc.citation.volume141-
dc.citation.issue16-
dc.citation.beginningpage3222-
dc.citation.endingpage3232-
dc.citation.publicationnameDEVELOPMENT-
dc.identifier.doi10.1242/dev.106229-
dc.contributor.localauthorChoi, Kwang-Wook-
dc.contributor.nonIdAuthorLinh Thuong Vuong-
dc.contributor.nonIdAuthorMukhopadhyay, Bibhash-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorArmadillo-
dc.subject.keywordAuthorDishevelled-
dc.subject.keywordAuthorDrosophila kinesin II-
dc.subject.keywordAuthorWingless/Wnt signaling-
dc.subject.keywordPlusBETA-CATENIN-
dc.subject.keywordPlusCHOLINE-ACETYLTRANSFERASE-
dc.subject.keywordPlusINTRACELLULAR-TRANSPORT-
dc.subject.keywordPlusEMBRYONIC EPIDERMIS-
dc.subject.keywordPlusCUT EXPRESSION-
dc.subject.keywordPlusCELL-ADHESION-
dc.subject.keywordPlusIN-VIVO-
dc.subject.keywordPlusGENE-
dc.subject.keywordPlusPROTEIN-
dc.subject.keywordPlusNOTCH-
Appears in Collection
BS-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 9 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0