DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ha, Yonghwang | ko |
dc.contributor.author | Yang, Aerin | ko |
dc.contributor.author | Lee, Seyoung | ko |
dc.contributor.author | Kim, Kibong | ko |
dc.contributor.author | Liew, Hyunjeong | ko |
dc.contributor.author | Suh, Yoo-Hun | ko |
dc.contributor.author | Park, Hee-Sung | ko |
dc.contributor.author | Churchill, David G | ko |
dc.date.accessioned | 2014-09-02T01:14:39Z | - |
dc.date.available | 2014-09-02T01:14:39Z | - |
dc.date.created | 2014-02-10 | - |
dc.date.created | 2014-02-10 | - |
dc.date.issued | 2014-01 | - |
dc.identifier.citation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.443, no.3, pp.1085 - 1091 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | http://hdl.handle.net/10203/189735 | - |
dc.description.abstract | Herein, a new method for preparing phosphorylated proteins at specific sites has been applied to alpha-synuclein (alpha-Syn). Three different alpha-Syn species phosphorylated at Serine 87 (S87p-alpha-Syn), Serine 129 (S129p-alpha-Syn) and Serine 87/129 (S87p,129p-alpha-Syn) were prepared through the 'stop codon' method and verified by LC/MS/MS and immunoblotting. Each type of phosphorylated alpha-Syn was tested for oligomerization trends and cellular toxicity with dopamine (DA), Cu2+ ions and pyridoxal 5'-phosphate. Aggregation trends induced by DA or DA/Cu2+ were similar between phosphorylated and non-phosphorylated alpha-Syn in SDS-PAGE. However, except for the monomer, phosphorylated oligomers showed higher toxicity than the non-phosphorylated alpha-Syn (Np-alpha-Syn) oligomers via WST-1 assays when tested on SH-SY5Y human neuroblastoma cells. In particular, S87p-alpha-Syn and S87p,129p-alpha-Syn oligomers induced by DA/Cu2+, showed higher toxicity than did S129p-alpha-Syn. When alpha-Syn was treated with pyridoxal 5'-phosphate in the presence of DA or Cu2+ to determine aggregation effects, high inhibition effects were shown in both non-phosphorylated and phosphorylated versions. alpha-Syn co-incubated with DA or DA/Cu2+ showed less cellular toxicity upon pyridoxal 5'-phosphate treatment, especially in the case of DA-induced Np-alpha-Syn. This study supports that phosphorylated oligomers of alpha-Syn at residue 87 can contribute to neuronal toxicity and the pyridoxal 5'-phosphate can be used as an inhibitor for alpha-Syn aggregation. (C) 2013 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.subject | ALPHA-SYNUCLEIN PHOSPHORYLATION | - |
dc.subject | PARKINSONS-DISEASE | - |
dc.subject | INCLUSION FORMATION | - |
dc.subject | CELL-DEATH | - |
dc.subject | AGGREGATION | - |
dc.subject | DOPAMINE | - |
dc.subject | NEUROTOXICITY | - |
dc.subject | PHOSPHOSERINE | - |
dc.subject | PATHOGENESIS | - |
dc.subject | ALDEHYDE | - |
dc.title | Facile "stop codon" method reveals elevated neuronal toxicity by discrete S87p-alpha-synuclein oligomers | - |
dc.type | Article | - |
dc.identifier.wosid | 000331415000050 | - |
dc.identifier.scopusid | 2-s2.0-84893697183 | - |
dc.type.rims | ART | - |
dc.citation.volume | 443 | - |
dc.citation.issue | 3 | - |
dc.citation.beginningpage | 1085 | - |
dc.citation.endingpage | 1091 | - |
dc.citation.publicationname | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.identifier.doi | 10.1016/j.bbrc.2013.12.099 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Park, Hee-Sung | - |
dc.contributor.localauthor | Churchill, David G | - |
dc.contributor.nonIdAuthor | Lee, Seyoung | - |
dc.contributor.nonIdAuthor | Kim, Kibong | - |
dc.contributor.nonIdAuthor | Liew, Hyunjeong | - |
dc.contributor.nonIdAuthor | Suh, Yoo-Hun | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Stop codon | - |
dc.subject.keywordAuthor | Phosphorylation | - |
dc.subject.keywordAuthor | Oligomerization | - |
dc.subject.keywordAuthor | Pyridoxal 5 &apos | - |
dc.subject.keywordAuthor | -phosphate | - |
dc.subject.keywordAuthor | Copper | - |
dc.subject.keywordAuthor | Dopamine | - |
dc.subject.keywordPlus | ALPHA-SYNUCLEIN PHOSPHORYLATION | - |
dc.subject.keywordPlus | PARKINSONS-DISEASE | - |
dc.subject.keywordPlus | INCLUSION FORMATION | - |
dc.subject.keywordPlus | CELL-DEATH | - |
dc.subject.keywordPlus | AGGREGATION | - |
dc.subject.keywordPlus | DOPAMINE | - |
dc.subject.keywordPlus | NEUROTOXICITY | - |
dc.subject.keywordPlus | PHOSPHOSERINE | - |
dc.subject.keywordPlus | PATHOGENESIS | - |
dc.subject.keywordPlus | ALDEHYDE | - |
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