Patterning biological molecules onto poly(amidoamine) dendrimer on gold and glass

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Patterning of biological molecules was attempted on both gold and glass using fourth generation (G4) poly(amidoamine) (PAMAM) dendrimer as an interfacing layer between solid surfaces and biomolecules. As for the patterning of avidin and anti-biotin antibody on gold, PAMAM dendrimers representing amine functionalities were firstly printed onto the 11-mercaptoundecanoic acid SAM by microcontact printing, followed by biotinylation, and reacted with fluorescence-labeled avidin or anti-biotin antibody. Fluorescence microscopic analysis revealed that the patterns of avidin and anti-biotin antibody were well constructed with the resolution of <2mum. The PAMAM dendrimers were also printed onto aldehyde-activated slide glass and reacted directly with anti-BSA antibodies, which had been oxidized with sodium periodate. As a result, distinct patterns of the anti-BSA antibodies were also obtained with a comparable edge resolution to that of avidin patterns on gold. These results clearly show that PAMAM dendrimers can be adopted as an interfacing layer for the patterning of biological molecules on solid surfaces with micrometer resolution.
Publisher
KOREAN CHEMICAL SOC
Issue Date
2003-08
Language
English
Article Type
Article
Keywords

SELF-ASSEMBLED MONOLAYERS; AFFINITY-SENSING SURFACE; STARBURST DENDRIMERS; THIN-FILMS; PROTEINS; CONSTRUCTION; BIOSENSOR; ANTIBODY; AVIDIN; FUNCTIONALIZATION

Citation

BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.24, pp.1197 - 1202

ISSN
0253-2964
URI
http://hdl.handle.net/10203/18142
Appears in Collection
BS-Journal Papers(저널논문)
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