Propionyl-CoA dependent biosynthesis of 2-hydroxybutyrate containing polyhydroxyalkanoates in metabolically engineered Escherichia coli

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dc.contributor.authorPark, Si Jaeko
dc.contributor.authorKang, Kyoung-Heeko
dc.contributor.authorLee, Hyukko
dc.contributor.authorPark, A-Reumko
dc.contributor.authorYang, Jung Eunko
dc.contributor.authorOh, Young Hoonko
dc.contributor.authorSong, Bong Keunko
dc.contributor.authorJegal, Jonggeonko
dc.contributor.authorLee, Seung Hwanko
dc.contributor.authorLee, SangYupko
dc.date.accessioned2013-07-18T07:01:15Z-
dc.date.available2013-07-18T07:01:15Z-
dc.date.created2013-07-03-
dc.date.created2013-07-03-
dc.date.created2013-07-03-
dc.date.issued2013-05-
dc.identifier.citationJOURNAL OF BIOTECHNOLOGY, v.165, no.2, pp.93 - 98-
dc.identifier.issn0168-1656-
dc.identifier.urihttp://hdl.handle.net/10203/174055-
dc.description.abstractWe have previously reported in vivo biosynthesis of 2-hydroxyacid containing polyesters including polylactic acid (PLA), poly(3-hydroxybutyrate-co-lactate) [P(3HB-co-LA)], and poly(3-hydroxybutyrate-co-2-hydroxybutyrate-co-lactate) [P(3HB-co-2HB-co-LA)] employing metabolically engineered Escherichia coil strains by the introduction of evolved Clostridium propionicum propionyl-CoA transferase (Pct(Cp)) and Pseudomonas sp. MBEL 6-19 polyhydroxyalkanoate (PHA) synthase I (PhaC1(Ps6-19)). In this study, we further engineered in vivo PLA biosynthesis system in E. coil to synthesize 2HB-containing PHA, in which propionyl-CoA was used as precursor for 2-ketobutyrate that was converted into 2HB-00A by the sequential actions of Lactococcus lactis (a)-2-hydroxybutyrate dehydrogenase (PanE) and Pct(Cp) and then 2HB-00A was polymerized by PhaC1(Ps6-19). The recombinant E. coli XL1-blue expressing the phaC1437 gene, the pct540 gene, and the Ralstonia eutropha prpE gene together with the panE gene could be grown to 0.66 g/L and successfully produced P(70 mol%3HB-co-18 mol%2HB-co-12 mol%LA) up to the PHA content of 66 wt% from 20 g/L of glucose, 2 g/L of 3 HB and 1 g/L of sodium propionate. Removal of the prpC gene in the chromosome of E. coli XLI -blue could increase the mole fraction of 2HB in copolymer, but the PHA content was decreased. The metabolic engineering strategy reported here suggests that propionyl-CoA can be successfully used as the precursor to provide PHA synthase with 2HB-CoA for the production of PHAs containing 2HB monomer. (C) 2013 Elsevier B.V. All rights reserved.-
dc.languageEnglish-
dc.publisherELSEVIER SCIENCE BV-
dc.titlePropionyl-CoA dependent biosynthesis of 2-hydroxybutyrate containing polyhydroxyalkanoates in metabolically engineered Escherichia coli-
dc.typeArticle-
dc.identifier.wosid000319244000003-
dc.identifier.scopusid2-s2.0-84876729194-
dc.type.rimsART-
dc.citation.volume165-
dc.citation.issue2-
dc.citation.beginningpage93-
dc.citation.endingpage98-
dc.citation.publicationnameJOURNAL OF BIOTECHNOLOGY-
dc.identifier.doi10.1016/j.jbiotec.2013.03.005-
dc.contributor.localauthorLee, SangYup-
dc.contributor.nonIdAuthorPark, Si Jae-
dc.contributor.nonIdAuthorKang, Kyoung-Hee-
dc.contributor.nonIdAuthorLee, Hyuk-
dc.contributor.nonIdAuthorPark, A-Reum-
dc.contributor.nonIdAuthorYang, Jung Eun-
dc.contributor.nonIdAuthorOh, Young Hoon-
dc.contributor.nonIdAuthorSong, Bong Keun-
dc.contributor.nonIdAuthorJegal, Jonggeon-
dc.contributor.nonIdAuthorLee, Seung Hwan-
dc.type.journalArticleArticle-
dc.subject.keywordAuthor2HB containing PHA-
dc.subject.keywordAuthorPropionyl-CoA-
dc.subject.keywordAuthor2-Ketobutyrate-
dc.subject.keywordAuthorMetabolic engineering-
dc.subject.keywordPlusUNRELATED CARBON-SOURCES-
dc.subject.keywordPlusFED-BATCH CULTURE-
dc.subject.keywordPlusBACTERIAL POLYHYDROXYALKANOATES-
dc.subject.keywordPlusPOLYLACTIC ACID-
dc.subject.keywordPlusPOLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE)-
dc.subject.keywordPlus1-PROPANOL-
dc.subject.keywordPlusPOLYESTERS-
dc.subject.keywordPlusCOPOLYMERS-
dc.subject.keywordPlusSYNTHASES-
dc.subject.keywordPlusBIOFUELS-
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