DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Si Jae | ko |
dc.contributor.author | Kang, Kyoung-Hee | ko |
dc.contributor.author | Lee, Hyuk | ko |
dc.contributor.author | Park, A-Reum | ko |
dc.contributor.author | Yang, Jung Eun | ko |
dc.contributor.author | Oh, Young Hoon | ko |
dc.contributor.author | Song, Bong Keun | ko |
dc.contributor.author | Jegal, Jonggeon | ko |
dc.contributor.author | Lee, Seung Hwan | ko |
dc.contributor.author | Lee, SangYup | ko |
dc.date.accessioned | 2013-07-18T07:01:15Z | - |
dc.date.available | 2013-07-18T07:01:15Z | - |
dc.date.created | 2013-07-03 | - |
dc.date.created | 2013-07-03 | - |
dc.date.created | 2013-07-03 | - |
dc.date.issued | 2013-05 | - |
dc.identifier.citation | JOURNAL OF BIOTECHNOLOGY, v.165, no.2, pp.93 - 98 | - |
dc.identifier.issn | 0168-1656 | - |
dc.identifier.uri | http://hdl.handle.net/10203/174055 | - |
dc.description.abstract | We have previously reported in vivo biosynthesis of 2-hydroxyacid containing polyesters including polylactic acid (PLA), poly(3-hydroxybutyrate-co-lactate) [P(3HB-co-LA)], and poly(3-hydroxybutyrate-co-2-hydroxybutyrate-co-lactate) [P(3HB-co-2HB-co-LA)] employing metabolically engineered Escherichia coil strains by the introduction of evolved Clostridium propionicum propionyl-CoA transferase (Pct(Cp)) and Pseudomonas sp. MBEL 6-19 polyhydroxyalkanoate (PHA) synthase I (PhaC1(Ps6-19)). In this study, we further engineered in vivo PLA biosynthesis system in E. coil to synthesize 2HB-containing PHA, in which propionyl-CoA was used as precursor for 2-ketobutyrate that was converted into 2HB-00A by the sequential actions of Lactococcus lactis (a)-2-hydroxybutyrate dehydrogenase (PanE) and Pct(Cp) and then 2HB-00A was polymerized by PhaC1(Ps6-19). The recombinant E. coli XL1-blue expressing the phaC1437 gene, the pct540 gene, and the Ralstonia eutropha prpE gene together with the panE gene could be grown to 0.66 g/L and successfully produced P(70 mol%3HB-co-18 mol%2HB-co-12 mol%LA) up to the PHA content of 66 wt% from 20 g/L of glucose, 2 g/L of 3 HB and 1 g/L of sodium propionate. Removal of the prpC gene in the chromosome of E. coli XLI -blue could increase the mole fraction of 2HB in copolymer, but the PHA content was decreased. The metabolic engineering strategy reported here suggests that propionyl-CoA can be successfully used as the precursor to provide PHA synthase with 2HB-CoA for the production of PHAs containing 2HB monomer. (C) 2013 Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.title | Propionyl-CoA dependent biosynthesis of 2-hydroxybutyrate containing polyhydroxyalkanoates in metabolically engineered Escherichia coli | - |
dc.type | Article | - |
dc.identifier.wosid | 000319244000003 | - |
dc.identifier.scopusid | 2-s2.0-84876729194 | - |
dc.type.rims | ART | - |
dc.citation.volume | 165 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 93 | - |
dc.citation.endingpage | 98 | - |
dc.citation.publicationname | JOURNAL OF BIOTECHNOLOGY | - |
dc.identifier.doi | 10.1016/j.jbiotec.2013.03.005 | - |
dc.contributor.localauthor | Lee, SangYup | - |
dc.contributor.nonIdAuthor | Park, Si Jae | - |
dc.contributor.nonIdAuthor | Kang, Kyoung-Hee | - |
dc.contributor.nonIdAuthor | Lee, Hyuk | - |
dc.contributor.nonIdAuthor | Park, A-Reum | - |
dc.contributor.nonIdAuthor | Yang, Jung Eun | - |
dc.contributor.nonIdAuthor | Oh, Young Hoon | - |
dc.contributor.nonIdAuthor | Song, Bong Keun | - |
dc.contributor.nonIdAuthor | Jegal, Jonggeon | - |
dc.contributor.nonIdAuthor | Lee, Seung Hwan | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | 2HB containing PHA | - |
dc.subject.keywordAuthor | Propionyl-CoA | - |
dc.subject.keywordAuthor | 2-Ketobutyrate | - |
dc.subject.keywordAuthor | Metabolic engineering | - |
dc.subject.keywordPlus | UNRELATED CARBON-SOURCES | - |
dc.subject.keywordPlus | FED-BATCH CULTURE | - |
dc.subject.keywordPlus | BACTERIAL POLYHYDROXYALKANOATES | - |
dc.subject.keywordPlus | POLYLACTIC ACID | - |
dc.subject.keywordPlus | POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE) | - |
dc.subject.keywordPlus | 1-PROPANOL | - |
dc.subject.keywordPlus | POLYESTERS | - |
dc.subject.keywordPlus | COPOLYMERS | - |
dc.subject.keywordPlus | SYNTHASES | - |
dc.subject.keywordPlus | BIOFUELS | - |
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