Chemical Imaging Analysis of the Micropatterns of Proteins and Cells Using Cluster Ion Beam-based Time-of-Flight Secondary Ion Mass Spectrometry and Principal Component Analysis

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Micropatterns of streptavidin and human epidermal carcinoma A431 cells were successfully imaged, as received and without any labeling, using cluster Au-3(+) ion beam-based time-of-flight secondary ion mass spectrometry (TOF-SIMS) together with a principal component analysis (PCA). Three different analysis ion beams (Ga+, Au+ and Au-3(+)) were compared to obtain label-free TOF-SIMS chemical images of micropatterns of streptavidin, which were subsequently used for generating cell patterns. The image of the total positive ions obtained by the Au-3(+) primary ion beam corresponded to the actual image of micropatterns of streptavidin, whereas the total positive-ion images by Ga+ or Au+ primary ion beams did not. A PCA of the TOF-SIMS spectra was initially performed to identify characteristic secondary ions of streptavidin. Chemical images of each characteristic ion were reconstructed from the raw data and used in the second PCA run, which resulted in a contrasted - and corrected - image of the micropatterns of streptavidin by the Ga+ and Au+ ion beams. The findings herein suggest that using cluster-ion analysis beams and multivariate data analysis for TOF-SIMS chemical imaging would be an effectual method for producing label-free chemical images of micropatterns of biomolecules, including proteins and cells.
Publisher
KOREAN CHEMICAL SOC
Issue Date
2013-03
Language
English
Article Type
Article
Keywords

SELF-ASSEMBLED MONOLAYERS; ORGANIC THIN-FILMS; BOMBARDMENT; FABRICATION; SURFACES; TISSUE; SIMS

Citation

BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.34, no.3, pp.815 - 819

ISSN
0253-2964
DOI
10.5012/bkcs.2013.34.3.815
URI
http://hdl.handle.net/10203/173874
Appears in Collection
CH-Journal Papers(저널논문)
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