Heterologous Expression of a Putative K+/H+ Antiporter of S. coelicolor A3(2) Enhances K+, Acidic-pH Shock Tolerances, and Geldanamycin Secretion

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dc.contributor.authorSong, Jae Yangko
dc.contributor.authorSeo, Young Binko
dc.contributor.authorHong, Soon-Kwangko
dc.contributor.authorChang, YongKeunko
dc.date.accessioned2013-04-11T07:50:21Z-
dc.date.available2013-04-11T07:50:21Z-
dc.date.created2013-04-02-
dc.date.created2013-04-02-
dc.date.issued2013-02-
dc.identifier.citationJOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.23, no.2, pp.149 - 155-
dc.identifier.issn1017-7825-
dc.identifier.urihttp://hdl.handle.net/10203/173462-
dc.description.abstractHeterologous expression of a putative K+/H+ antiporter of Streptomyces coelicolor A3(2) (designated as sha4) in E. coli and Streptomyces hygroscopicus JCM4427 showed enhanced tolerance to K+ stress, acidic-pH shock, and/or geldanamycin production under K+ stress. In a series of K+ extrusion experiments with sha4-carrying E. coli deficient in the K+/H+ antiporter, a restoration of impaired K+ extrusion activity was observed. Based on this, it was concluded that sha4 was a true K+/H+ antiporter. In different sets of experiments, the sha4-carrying E. coil showed significantly improved tolerances to K+ stresses and acidic-pH shock, whereas sha4-carrying S. hygroscopicus showed an improvement in K+ stress tolerance only. The sha4-carrying S. hygroscopicus showed much higher geldanamycin productivity than the control under K+ stress condition. In another set of experiments with a production medium, the secretion of geldanamycin was also significantly enhanced by the expression of sha4.-
dc.languageEnglish-
dc.publisherKOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY-
dc.subjectNA+/H+ ANTIPORTER-
dc.subjectESCHERICHIA-COLI-
dc.subjectBACILLUS-SUBTILIS-
dc.subjectSALT-STRESS-
dc.subjectHELICOBACTER-PYLORI-
dc.subjectSTREPTOMYCES-
dc.subjectOVEREXPRESSION-
dc.subjectARABIDOPSIS-
dc.subjectH+-
dc.subjectTRANSPORTERS-
dc.titleHeterologous Expression of a Putative K+/H+ Antiporter of S. coelicolor A3(2) Enhances K+, Acidic-pH Shock Tolerances, and Geldanamycin Secretion-
dc.typeArticle-
dc.identifier.wosid000315547800003-
dc.identifier.scopusid2-s2.0-84873823605-
dc.type.rimsART-
dc.citation.volume23-
dc.citation.issue2-
dc.citation.beginningpage149-
dc.citation.endingpage155-
dc.citation.publicationnameJOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY-
dc.identifier.doi10.4014/jmb.1207.07030-
dc.contributor.localauthorChang, YongKeun-
dc.contributor.nonIdAuthorSong, Jae Yang-
dc.contributor.nonIdAuthorSeo, Young Bin-
dc.contributor.nonIdAuthorHong, Soon-Kwang-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorK+/H+ antiporter-
dc.subject.keywordAuthorsalt tolerance-
dc.subject.keywordAuthorpH shock tolerance-
dc.subject.keywordAuthorgeldanamycin-
dc.subject.keywordPlusNA+/H+ ANTIPORTER-
dc.subject.keywordPlusESCHERICHIA-COLI-
dc.subject.keywordPlusBACILLUS-SUBTILIS-
dc.subject.keywordPlusSALT-STRESS-
dc.subject.keywordPlusHELICOBACTER-PYLORI-
dc.subject.keywordPlusSTREPTOMYCES-
dc.subject.keywordPlusOVEREXPRESSION-
dc.subject.keywordPlusARABIDOPSIS-
dc.subject.keywordPlusH+-
dc.subject.keywordPlusTRANSPORTERS-
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