Mutations upregulating the flhDC operon of Escherichia coli K-12

Cited 16 time in webofscience Cited 0 time in scopus
  • Hit : 394
  • Download : 0
Bacterial motility is governed by the flhDC master operon that is under the control of factors like OmpR, LrhA, HdfR, and H-NS. Previously, derivatives of the wild-type MG1655 strain of E. coli K-12 with enhanced motility were found to contain insertion sequences (ISs) in the regulatory region of the flhDC operon. Here, we report that not only integrations of IS insertion sequences into the regulatory region of the flhDC operon, but also a missense mutation in the lrhA gene enhances motility by relieving transcriptional repression of the flhDC operon. Two novel IS insertions were found upstream of flhDC. So far, the relationships between the trans- acting factors and the cis-acting regulatory sequences associated with the flhDC operon have not been clearly established. In this study, it was found that effects of the cis- and trans-acting mutations were acting in parallel, suggesting their apparently independent regulation of flagellar expression.
Publisher
MICROBIOLOGICAL SOCIETY KOREA
Issue Date
2013-02
Language
English
Article Type
Article
Keywords

H-NS PROTEIN; SALMONELLA-TYPHIMURIUM; CHEMOTAXIS GENES; FLAGELLAR MOTOR; CO2 FIXATION; LYSR FAMILY; INSERTION; EXPRESSION; MOTILITY; TRANSCRIPTION

Citation

JOURNAL OF MICROBIOLOGY, v.51, no.1, pp.140 - 144

ISSN
1225-8873
DOI
10.1007/s12275-013-2212-z
URI
http://hdl.handle.net/10203/173461
Appears in Collection
BS-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 16 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0