SERS-based Identification of Pathogenic Bacteria Using Patterned Au Particle-on-wire Sensor

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As surface-enhanced Raman scattering (SERS) is a convenient and sensitive method that can detect the analytes even on a single molecule level using a simple nanostructure, it has been employing as an attractive strategy for sensitive DNA sensing. Here, we report the development of a multiplex bacterial DNA detection method employing Au particleon-wire systems as a SERS sensing platform1. The Au NW-on-wire exhibits superb reproducibility, excellent sensitivity, and good time stability. A pattern formed by multiplex Au NW sensors provides positional address and identification for each sensor. By using this system, multiplex target DNAs were detected with a detection limit of 10 pM in a quantitative manner. The target DNAs obtained from reference strains and clinical samples were identified by the particle-on-wire SERS sensor developed in this study successfully, predicting practical values of this sensor in a variety of biosensing application where DNA binding involved.
Publisher
한국생물공학회
Issue Date
2010-10-07
Language
Korean
Citation

2010 KSBB Fall Meeting and International Symposium

URI
http://hdl.handle.net/10203/171666
Appears in Collection
CH-Conference Papers(학술회의논문)CBE-Conference Papers(학술회의논문)
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