DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김지만 | ko |
dc.contributor.author | 이상엽 | ko |
dc.date.accessioned | 2013-03-28T04:51:06Z | - |
dc.date.available | 2013-03-28T04:51:06Z | - |
dc.date.created | 2012-07-05 | - |
dc.date.created | 2012-07-05 | - |
dc.date.issued | 2008-04 | - |
dc.identifier.citation | Annual Spring Meeting of KIChE | - |
dc.identifier.uri | http://hdl.handle.net/10203/162925 | - |
dc.description.abstract | A markerless gene deletion method was developed to generate genetically modified Mannheimia succiniciproducens, which is a rumen bacterium producing succinic acid as a major metabolite. Cre recombinase was transiently expressed using a temperature sensitive plasmid to excise the antibiotic marker between the mutant lox sites. As a demonstration of the method, ldhA gene and oadGAB operon was sequentially deleted without leaving an antibiotic marker inside the genome of M. succiniciproducens. | - |
dc.language | Korean | - |
dc.publisher | 한국화학공학회 | - |
dc.title | Multiple gene deletion of Mannheimia succiniciproducens using mutant lox sequences | - |
dc.type | Conference | - |
dc.type.rims | CONF | - |
dc.citation.publicationname | Annual Spring Meeting of KIChE | - |
dc.identifier.conferencecountry | KO | - |
dc.identifier.conferencelocation | Jeju island | - |
dc.contributor.localauthor | 이상엽 | - |
dc.contributor.nonIdAuthor | 김지만 | - |
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