DC Field | Value | Language |
---|---|---|
dc.contributor.author | Noh, JM | - |
dc.contributor.author | Sung, SW | - |
dc.contributor.author | Jeon, MK | - |
dc.contributor.author | Kim, SH | - |
dc.contributor.author | Lee, L.P. | - |
dc.contributor.author | Woo, Seong-Ihl | - |
dc.date.accessioned | 2013-03-18T21:00:09Z | - |
dc.date.available | 2013-03-18T21:00:09Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2004-12-01 | - |
dc.identifier.citation | Symposium on biochip and nano-micro system for life science & chemistry, v., no., pp.0 - 0 | - |
dc.identifier.uri | http://hdl.handle.net/10203/152399 | - |
dc.description.abstract | A simple, reliable liquid crystal (LC) thermometry of a polymerase chain reaction (PCR) chip that has a microchamber inside the chip and a film heater and a film sensor patterned on the surface is firstly introduced. The silicon-based PCR chip is made by MEMS technology. A non-linear feedback proportional-integral control scheme adjusts the power input of the Pt heater to control the surface temperature of the micro-PCR chip measured by the Pt sensor with an accuracy of less than ±0.1 °C at steady state. We use the LC thermometry to analyze the temperature distribution in the microchamber of the micro-PCR chip. The reflected colors of LC's inside the microchamber are captured through a charge-coupled device (CCD) camera, subsequently analyzed using a hue-based color analysis system. Two LC's of which individual working range is near the typical PCR temperature of 55 °C and 72 °C are calibrated in a constant temperature bath with a resolution of less than ±0.3 °C. While the average microchamber temperature near the annealing temperature is similar to the surface temperature, it is lower than the surface temperature by ca. 1 °C in the range of the extension temperature. Also, the temperature variations along the center of the microchamber, are less than 1.5 ± 0.5 °C and 3 ± 0.3 °C in the range of the annealing and the extension temperature, respectively. | - |
dc.language | ENG | - |
dc.title | In situ thermal diagnotics of the micro-PCR system using liquid crystals | - |
dc.type | Conference | - |
dc.type.rims | CONF | - |
dc.citation.beginningpage | 0 | - |
dc.citation.endingpage | 0 | - |
dc.citation.publicationname | Symposium on biochip and nano-micro system for life science & chemistry | - |
dc.identifier.conferencecountry | South Korea | - |
dc.identifier.conferencecountry | South Korea | - |
dc.contributor.localauthor | Woo, Seong-Ihl | - |
dc.contributor.nonIdAuthor | Noh, JM | - |
dc.contributor.nonIdAuthor | Sung, SW | - |
dc.contributor.nonIdAuthor | Jeon, MK | - |
dc.contributor.nonIdAuthor | Kim, SH | - |
dc.contributor.nonIdAuthor | Lee, L.P. | - |
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