Reversible affinity interactions of antibody molecules at functionalized dendrimer monolayer: affinity-sensing surface with reusability

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We described reversible affinity interactions of antibody molecules at a chemically functionalized electrode surface for a repeatedly renewable affinity-biosensing interface. Underlying biofunctionalizable monolayers were constructed with poly(amidoamine) dendrimers, whose surface chain-end groups were double-functionalized with biotinyl ligand and ferrocenyl groups for biospecific recognition and electron transfer reactions, respectively. Functionalized monolayers on gold electrodes provide platform surfaces for biospecific recognition reaction with monoclonal anti-biotin antibody molecules. Bound antibody molecules were dissociated from the surface via displacement reaction by the addition of free biotin in solution, enabling the affinity surface to be renewed and repeatedly utilized. Tracking of the association/dissociation reaction cycles were performed by registering the bioelectrocatalytic currents at the electrode using glucose oxidase (GOx) as a signal generator and ferrocenyl-tethered dendrimer (Fc-D) as an electron transferring mediator in electrolyte. Shielding of the affinity surface by biospecifically bound antibody molecules caused hindrance in electron transfer, resulting in reduced signal from cyclic voltammetry. By the displacement reaction using free biotin, bound antibody molecules were dissociated from the surface and the bioelectrocatalytic signal was restored. With the affinity surfaces constructed in this work, continuous association/dissociation reactions have been successfully accomplished, providing a possibility of reusable affinity biosensing inter-face. (C) 2002 Elsevier Science B.V. All rights reserved.
Publisher
ELSEVIER SCIENCE BV
Issue Date
2002-04
Language
English
Article Type
Article
Keywords

SELF-ASSEMBLED MONOLAYERS; GOLD; ELECTRODES; BIOSENSOR; PROTEINS; BINDING; LIGANDS; AVIDIN; BIOTIN; CONSTRUCTION

Citation

ANALYTICA CHIMICA ACTA, v.456, no.2, pp.209 - 218

ISSN
0003-2670
URI
http://hdl.handle.net/10203/14246
Appears in Collection
BS-Journal Papers(저널논문)
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