DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cho, KC | ko |
dc.contributor.author | Jeong, JH | ko |
dc.contributor.author | Chung, Hyun Jung | ko |
dc.contributor.author | Joe, Cheol O | ko |
dc.contributor.author | Kim, SW | ko |
dc.contributor.author | Park, Tae Gwan | ko |
dc.date.accessioned | 2009-11-11T05:26:04Z | - |
dc.date.available | 2009-11-11T05:26:04Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2005-11 | - |
dc.identifier.citation | JOURNAL OF CONTROLLED RELEASE, v.108, no.1, pp.121 - 131 | - |
dc.identifier.issn | 0168-3659 | - |
dc.identifier.uri | http://hdl.handle.net/10203/12414 | - |
dc.description.abstract | R ecombinant reversed caspase-3 (rev-caspase-3) is a pro-apoptotic gene capable of intracellular autocatalytic processing, which leads to programmed cell death. Folate receptor-specific intracellular delivery of the rev-caspase-3 gene into KB cells overexpressing folate receptors was explored by employing the folate-poly(ethylene glycol)-polyethylenimine (FOL-PEG-PEI) conjugate as a nonviral polymeric carrier. Using luciferase as a reporter gene, the conditions for formulation of DNA/polymer polyplexes were pre-optimized to attain the highest folate receptor-mediated gene transfection efficiency. FOL-PEG-PEI conjugate complexed with rev-caspase-3 plasmid in an optimized condition gave rise to a great increase in expression and activation of exogenous rev-caspase-3 in KB cells when pretreated with doxorubicin. The synthesized conjugate exhibited higher transfection efficiency than other commercially available transfection agents due to a unique mechanism of folate-receptor mediated endocytic gene transfer. The transfected cells showed a significant extent of apoptosis by rev-caspase-3. This study suggests the potential of using folate-receptor-mediated delivery of rev-caspase-3 gene for inducing tumor cell death in a target-specific manner. (c) 2005 Elsevier B.V All rights reserved. | - |
dc.description.sponsorship | National Cancer Institute (02-3-150) and from the Ministry of Science and Technology (M102140000117- 02b1500-02110), Republic of Korea. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | GENE DELIVERY | - |
dc.subject | CATIONIC POLYMERS | - |
dc.subject | PLASMID DNA | - |
dc.subject | CELLS | - |
dc.subject | CANCER | - |
dc.subject | POLYETHYLENIMINE | - |
dc.subject | BINDING | - |
dc.subject | GA-67-DEFEROXAMINE-FOLATE | - |
dc.subject | EXPRESSION | - |
dc.subject | COMPLEXES | - |
dc.title | Folate receptor-mediated intracellular delivery of recombinant caspase-3 for inducing apoptosis | - |
dc.type | Article | - |
dc.identifier.wosid | 000233321200012 | - |
dc.identifier.scopusid | 2-s2.0-26944466093 | - |
dc.type.rims | ART | - |
dc.citation.volume | 108 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 121 | - |
dc.citation.endingpage | 131 | - |
dc.citation.publicationname | JOURNAL OF CONTROLLED RELEASE | - |
dc.identifier.doi | 10.1016/j.jconrel.2005.07.015 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Chung, Hyun Jung | - |
dc.contributor.localauthor | Joe, Cheol O | - |
dc.contributor.localauthor | Park, Tae Gwan | - |
dc.contributor.nonIdAuthor | Cho, KC | - |
dc.contributor.nonIdAuthor | Jeong, JH | - |
dc.contributor.nonIdAuthor | Kim, SW | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | PEI | - |
dc.subject.keywordAuthor | folate | - |
dc.subject.keywordAuthor | receptor-mediated endocytosis | - |
dc.subject.keywordAuthor | rev-caspase-3 | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordPlus | GENE DELIVERY | - |
dc.subject.keywordPlus | CATIONIC POLYMERS | - |
dc.subject.keywordPlus | PLASMID DNA | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | POLYETHYLENIMINE | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | GA-67-DEFEROXAMINE-FOLATE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | COMPLEXES | - |
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