Facile Immobilization of Biomolecules onto Various Surfaces Using Epoxide-Containing Antibiofouling Polymers

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The surface modifications of plastic or glass substrate and the subsequent immobilization of biomolecules onto the surfaces has been a central feature of the fabrication of biochips. To this end, we designed and synthesized new epoxide-containing random copolymers that form stable polymer adlayers on plastic or glass surface and subsequently react with amine or sulfhydryl functional groups of biomolecules under aqueous conditions. Epoxide-containing random copolymers were synthesized by radical polymerization of three functional monomers: a monomer acting as an anchor to the surfaces, a PEG group for preventing nonspecific protein adsorption, and an epoxide group for conjugating to biomolecules. Polymer coating layers were facilely formed on cyclic olefin copolymer (COC) or glass substrate by simply dipping each substrate into a solution of each copolymer. The polymer-coated surfaces characterized by a contact angle analyzer and X-ray photoelectron spectroscopy (XPS) showed very low levels of nonspecific immunoglobulin G (IgG) adsorption compared to the uncoated bare surface (control). Using a microcontact printing (mu CP) method, antibodies as representative biomolecules could be selectively attached onto the copolymers-coated glass or COC surface with high signal-to-noise ratios.
Publisher
AMER CHEMICAL SOC
Issue Date
2012-03
Language
English
Article Type
Article
Keywords

SELF-ASSEMBLED MONOLAYERS; TIN OXIDE ELECTRODE; PERFLUOROPHENYL AZIDES; SOFT LITHOGRAPHY; CARBON NANOTUBE; PROTEINS; FUNCTIONALIZATION; CONSTRUCTION; COPOLYMER; PRECURSOR

Citation

LANGMUIR, v.28, no.9, pp.4507 - 4514

ISSN
0743-7463
DOI
10.1021/la204898y
URI
http://hdl.handle.net/10203/104203
Appears in Collection
BS-Journal Papers(저널논문)
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