DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Chan Joo | ko |
dc.contributor.author | Jung, Jae Hwan | ko |
dc.contributor.author | Seo, Tae Seok | ko |
dc.date.accessioned | 2013-03-13T01:30:00Z | - |
dc.date.available | 2013-03-13T01:30:00Z | - |
dc.date.created | 2012-07-06 | - |
dc.date.created | 2012-07-06 | - |
dc.date.issued | 2012-06 | - |
dc.identifier.citation | ANALYTICAL CHEMISTRY, v.84, no.11, pp.4928 - 4934 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.uri | http://hdl.handle.net/10203/104118 | - |
dc.description.abstract | In this study, we demonstrated an effective sample pretreatment microdevice that could perform the capture, purification, and release of pathogenic bacteria with a large-volume sample and at a high speed and high-capture yield. We integrated a sol gel matrix into the microdevice which forms three-dimensional (3D) micropores for the cell solution to pass through and provides a large surface area for the immobilization of antibodies to capture the target Staphylococcus aureus (S. aureus) cells. The antibody was linked to the surface of the sol gel via a photocleavable linker, allowing the cell-captured antibody moiety to be released by UV irradiation. In addition to the optimization of the antibody immobilization and UV cleavage processes, the cell-capture efficiency was maximized by controlling the sample flow rate with a pumping scheme (3 steps, 5 steps: 3 steps with one flutter step, 7 steps: 3 steps with two flutter steps) and the pumping time (100, 200, and 300 ms). A quantitative capture analysis was performed by targeting a specific gene site of protein A of S. aureus in real-time PCR (RT-PCR). While the 3-step process with an actuation time of 100 ms showed the fastest flow rate (1 mL sample processing time in 10 min), the pumping scheme with the 7-step process and the 300 ms actuation time revealed the highest cell-capture efficiency. A limit of detection study with the 7-step and the 300 ms pumping scheme demonstrated that 100 cells per 100 mu L were detected with a 70% yield, and even a single cell could be analyzed via on-chip sample preparation. Thus, our novel sol gel based microdevice was proven more cost-effective, simple, and efficient in terms of its sample pretreatment ability compared to the use of a conventional 2D flat microdevice. This proposed sample pretreatment device can be further incorporated to an analytical functional unit to realize a micrototal analysis system (mu TAS) with sample-in answer-out capability in the fields of biomedical diagnostics, food safety testing, and environmental pollutant screening. | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | POLYMERASE-CHAIN-REACTION | - |
dc.subject | REAL-TIME PCR | - |
dc.subject | CAPILLARY-ELECTROPHORESIS | - |
dc.subject | MICROFLUIDIC DEVICES | - |
dc.subject | PATHOGEN DETECTION | - |
dc.subject | VIRUS DETECTION | - |
dc.subject | NUCLEIC-ACIDS | - |
dc.subject | DNA | - |
dc.subject | CHIP | - |
dc.subject | EXTRACTION | - |
dc.title | 3D Porous Sol-Gel Matrix Incorporated Microdevice for Effective Large Volume Cell Sample Pretreatment | - |
dc.type | Article | - |
dc.identifier.wosid | 000304783100042 | - |
dc.identifier.scopusid | 2-s2.0-84861899368 | - |
dc.type.rims | ART | - |
dc.citation.volume | 84 | - |
dc.citation.issue | 11 | - |
dc.citation.beginningpage | 4928 | - |
dc.citation.endingpage | 4934 | - |
dc.citation.publicationname | ANALYTICAL CHEMISTRY | - |
dc.identifier.doi | 10.1021/ac3005549 | - |
dc.contributor.localauthor | Seo, Tae Seok | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | POLYMERASE-CHAIN-REACTION | - |
dc.subject.keywordPlus | REAL-TIME PCR | - |
dc.subject.keywordPlus | CAPILLARY-ELECTROPHORESIS | - |
dc.subject.keywordPlus | MICROFLUIDIC DEVICES | - |
dc.subject.keywordPlus | PATHOGEN DETECTION | - |
dc.subject.keywordPlus | VIRUS DETECTION | - |
dc.subject.keywordPlus | NUCLEIC-ACIDS | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | CHIP | - |
dc.subject.keywordPlus | EXTRACTION | - |
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