Formation of liposome by microfluidic flow focusing and its application in gene delivery

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We report the formation of liposomes in a simple procedure using a microfluidic hydrodynamic flow focusing method for the application in gene delivery. We fabricated microfluidic device using soft lithography and polydimethylsiloxane (PDMS) molding technique. Lipid-containing stream was surrounded by aqueous stream and liposomes were formed at the lipid-water interface. Size distribution of liposomes and zeta potential of liposome dispersion were investigated under various flow rate ratio (FRR) and processing temperature. Size distributions of liposomes were measured by dynamic light scattering (DLS), and zeta potential was measured to quantify the colloidal stability. Prepared liposomes were used as a vehicle for gene delivery, and the successful expression of delivered gene was observed by fluorescent microscope.
Publisher
KOREAN SOC RHEOLOGY
Issue Date
2012-06
Language
English
Article Type
Article
Keywords

PHOSPHOLIPID-COMPOSITION; VESICLES; ENTRAPMENT; SYSTEM; SIZE

Citation

KOREA-AUSTRALIA RHEOLOGY JOURNAL, v.24, no.2, pp.129 - 135

ISSN
1226-119X
DOI
10.1007/s13367-012-0015-0
URI
http://hdl.handle.net/10203/103185
Appears in Collection
CBE-Journal Papers(저널논문)
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