Increased immunogenicity of avian influenza DNA vaccine delivered to the skin using a microneedle patch

Cited 51 time in webofscience Cited 0 time in scopus
  • Hit : 237
  • Download : 0
Effective public health responses to an influenza pandemic require an effective vaccine that can be manufactured and administered to large populations in the shortest possible time. In this study, we evaluated a method for vaccination against avian influenza virus that uses a DNA vaccine for rapid manufacturing and delivered by a microneedle skin patch for simplified administration and increased immunogenicity. We prepared patches containing 700-mu m long microneedles coated with an avian H5 influenza hemagglutinin DNA vaccine from A/Viet Nam/1203/04 influenza virus. The coating DNA dose increased with DNA concentration in the coating solution and the number of dip-coating cycles. Coated DNA was released into the skin tissue by dissolution within minutes. Vaccination of mice using microneedles induced higher levels of antibody responses and hemagglutination inhibition titers, and improved protection against lethal infection with avian influenza as compared to conventional intramuscular delivery of the same dose of the DNA vaccine. Additional analysis showed that the microneedle coating solution containing carboxymethylcellulose and a surfactant may have negatively affected the immunogenicity of the DNA vaccine. Overall, this study shows that DNA vaccine delivery by microneedles can be a promising approach for improved vaccination to mitigate an influenza pandemic. (C) 2012 Elsevier B.V. All rights reserved.
Publisher
ELSEVIER SCIENCE BV
Issue Date
2012-06
Language
English
Article Type
Article
Keywords

VIRUS-LIKE PARTICLES; H5N1 VIRUSES; ANTIBODY-RESPONSES; INTRADERMAL VACCINATION; GENETIC IMMUNIZATION; PROTECTIVE IMMUNITY; CHALLENGE; ELECTROPORATION; INFECTION; HUMANS

Citation

EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, v.81, no.2, pp.239 - 247

ISSN
0939-6411
DOI
10.1016/j.ejpb.2012.03.010
URI
http://hdl.handle.net/10203/101855
Appears in Collection
CBE-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 51 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0