DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ju, Hye Kyung | ko |
dc.contributor.author | Hwang, Su-Jeong | ko |
dc.contributor.author | Jeon, Choon-Ju | ko |
dc.contributor.author | Lee, Gyun-Min | ko |
dc.contributor.author | Yoon, Sung Kwan | ko |
dc.date.accessioned | 2013-03-12T01:36:12Z | - |
dc.date.available | 2013-03-12T01:36:12Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2009-08 | - |
dc.identifier.citation | JOURNAL OF BIOTECHNOLOGY, v.143, no.2, pp.145 - 150 | - |
dc.identifier.issn | 0168-1656 | - |
dc.identifier.uri | http://hdl.handle.net/10203/100978 | - |
dc.description.abstract | To investigate the effect of hyperosmotic medium on production and aggregation of the variant of Angiopoietin-1 (Ang1), cartilage oligomeric matrix protein (COMP)-Ang1, in recombinant Chinese hamster ovary (CHO) cells, CHO cells were cultivated in shaking flasks. NaCl and/or sorbitol were used to raise medium osmolality in the range of 300-450mOsm/kg. The specific productivity of COMP-Ang1, q(COMP-Ang1), increased as medium osmolality increased. At NaCl-450 mOsm/kg, the q(COMP-Ang1) was 7.7-fold higher than that at NaCl-300 mOsm/kg, while, at sorbitol-450 mOsm/kg, it was 2.9-fold higher than that at sorbitol-300 mOsm/kg. This can be attributed to the increased relative mRNA level of COMP-Ang1 at NaCl-450 mOsm/kg which was approximately 2.4-fold higher than that at sorbitol-450 mOsm/kg. Western blot analysis showed that COMP-Ang1 aggregates started to occur in the late-exponential phase of cell growth. When sorbitol was used to raise the medium osmolality, a severe aggregation of COMP-Ang1 was observed. On the other hand, when NaCl was used, the aggregation of COMP-Ang1 was drastically reduced at NaCl-400 mOsm/kg. At NaCl-450 mOsm/kg, the aggregation of COMP-Ang1 was hardly observed. This suggests that environmental conditions are critical for the aggregation of COMP-Ang1. Taken together, the use of NaCl-incluced hyperosmotic medium to cell culture process turns out to be an efficient strategy for enhancing COMP-Ang1 production and reducing COMP-Ang1 aggregation. (C) 2009 Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | HYPEROSMOTIC PRESSURE | - |
dc.subject | PROTEIN AGGREGATION | - |
dc.subject | CHO-CELLS | - |
dc.subject | ANGIOPOIETIN-1 | - |
dc.subject | EXPRESSION | - |
dc.subject | COMP-ANG1 | - |
dc.subject | GROWTH | - |
dc.title | Use of NaCl prevents aggregation of recombinant COMP-Angiopoietin-1 in Chinese hamster ovary cells | - |
dc.type | Article | - |
dc.identifier.wosid | 000269850700010 | - |
dc.identifier.scopusid | 2-s2.0-68549086867 | - |
dc.type.rims | ART | - |
dc.citation.volume | 143 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 145 | - |
dc.citation.endingpage | 150 | - |
dc.citation.publicationname | JOURNAL OF BIOTECHNOLOGY | - |
dc.identifier.doi | 10.1016/j.jbiotec.2009.06.017 | - |
dc.contributor.localauthor | Lee, Gyun-Min | - |
dc.contributor.nonIdAuthor | Ju, Hye Kyung | - |
dc.contributor.nonIdAuthor | Jeon, Choon-Ju | - |
dc.contributor.nonIdAuthor | Yoon, Sung Kwan | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | CHO cells | - |
dc.subject.keywordAuthor | COMP-Ang1 | - |
dc.subject.keywordAuthor | Hyperosmotic medium | - |
dc.subject.keywordAuthor | Aggregation | - |
dc.subject.keywordAuthor | Specific COMP-Ang1 productivity | - |
dc.subject.keywordPlus | HYPEROSMOTIC PRESSURE | - |
dc.subject.keywordPlus | PROTEIN AGGREGATION | - |
dc.subject.keywordPlus | CHO-CELLS | - |
dc.subject.keywordPlus | ANGIOPOIETIN-1 | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | COMP-ANG1 | - |
dc.subject.keywordPlus | GROWTH | - |
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