DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jung, Jae Hee | ko |
dc.contributor.author | Lee, Jung Eun | ko |
dc.contributor.author | Kim, Sang Soo | ko |
dc.date.accessioned | 2009-07-16T09:52:33Z | - |
dc.date.available | 2009-07-16T09:52:33Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2009-04 | - |
dc.identifier.citation | ANALYTICAL CHEMISTRY, v.81, no.8, pp.2985 - 2990 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.uri | http://hdl.handle.net/10203/10081 | - |
dc.description.abstract | Biological electrospray techniques are rapidly becoming a promising means for controlling living organisms in applications ranging from mass spectrometry to developmental biology. We investigated the generation characteristics of airborne MS2 bacteriophage particles <30 nm in size, using an electrospray technique. A suspension containing bacteriophage MS2 was sprayed in cone-jet mode using a specially designed electrospray system with a point-to-orifice-plate configuration mentioned in previous studies based on a charge reduced electrospray size spectrometry. The highly charged droplets were discharged rapidly into a radioactive neutralizer of Po(210). The electrosprayed airborne MS2 particles (23.8 +/- 0.49 nm GMD) maintained their monodisperse size distribution with good stability and uniformity for > 1 h. Compared with the generation characteristics observed using the previous nebulization process (51.5 +/- 0.86 nm GMD), this electrospray technique produced nonagglomerated particles, resulting in a narrow size range of generated particles. The total MS2 particle number concentration and GMD increased with changes in the suspension flow rate from 5 to 25 mu L/h. As the applied voltage increased in cone-jet mode, the GMD and culturable bacteriophage concentration decreased slightly. Our investigation shows that the electrospray process, driven by high-intensity electric fields, can be used for nanometer-sized living organisms. | - |
dc.description.sponsorship | This work was supported by the Korea Advanced Institute of Science and Technology (KAIST) and the Brain Korea 21 Program of the South Korea Ministry of Education, Science, and Technology. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | CONE-JET MODE | - |
dc.subject | MOBILITY MOLECULAR ANALYZER | - |
dc.subject | MONODISPERSE WATER DROPLETS | - |
dc.subject | ELECTROPHORETIC MOBILITY | - |
dc.subject | MASS-SPECTROMETRY | - |
dc.subject | SCALING LAWS | - |
dc.subject | LIQUIDS | - |
dc.subject | VIRUS | - |
dc.subject | IONIZATION | - |
dc.subject | PROTEINS | - |
dc.title | Generation of Nonagglomerated Airborne Bacteriophage Particles Using an Electrospray Technique | - |
dc.type | Article | - |
dc.identifier.wosid | 000265158800020 | - |
dc.identifier.scopusid | 2-s2.0-65249166540 | - |
dc.type.rims | ART | - |
dc.citation.volume | 81 | - |
dc.citation.issue | 8 | - |
dc.citation.beginningpage | 2985 | - |
dc.citation.endingpage | 2990 | - |
dc.citation.publicationname | ANALYTICAL CHEMISTRY | - |
dc.identifier.doi | 10.1021/ac802584z | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Kim, Sang Soo | - |
dc.contributor.nonIdAuthor | Jung, Jae Hee | - |
dc.contributor.nonIdAuthor | Lee, Jung Eun | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | CONE-JET MODE | - |
dc.subject.keywordPlus | MOBILITY MOLECULAR ANALYZER | - |
dc.subject.keywordPlus | MONODISPERSE WATER DROPLETS | - |
dc.subject.keywordPlus | ELECTROPHORETIC MOBILITY | - |
dc.subject.keywordPlus | MASS-SPECTROMETRY | - |
dc.subject.keywordPlus | SCALING LAWS | - |
dc.subject.keywordPlus | LIQUIDS | - |
dc.subject.keywordPlus | VIRUS | - |
dc.subject.keywordPlus | IONIZATION | - |
dc.subject.keywordPlus | PROTEINS | - |
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