Patterned Immobilization of Biomolecules by Using Ion Irradiation-Induced Graft Polymerization

Abstract

A new method for biomolecular patterning based on ion irradiation-induced graft polymerization was demonstrated in this study. Ion irradiation on a polymer surface resulted in the formation of active species, which was further used for surface-initiated graft polymerization of acrylic acid. The results of the grafting study revealed that the surface graft polymerization using 20 vol % of acrylic acid on the poly(tetrafluoroethylene) (PTFE) film irradiated at the fluence of 1 x 10(15) ions/ cm 2 for 12 h was the optimum graft polymerization condition to achieve the maximum grafting degree. The results of the fluorescence microscopy also revealed that the optimum fluence to achieve the maximum fluorescence intensity was 1 x 101,5 ions/cm(2). The grafting of acrylic acid on the PTFE surfaces was confirmed by a fluorescence labeling method. The grafted PTFE films were used for the immobilization of amine-functionalized p-DNA, followed by hybridization with fluorescently tagged c-DNA. Biotin-amine was also immobilized on the acrylic acid grafted PTFE surfaces. Successful biotin-specific binding of streptavidin further confirmed the potential of this strategy for patterning of various biomolecules. (C) 2009 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 47: 6124-6134, 2009