In Vivo Measurement of Age-Related Stiffening in the Crystalline Lens by Brillouin Optical Microscopy

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The biophysical and biomechanical properties of the crystalline lens (e.g., viscoelasticity) have long been implicated in accommodation and vision problems, such as presbyopia and cataracts. However, it has been difficult to measure such parameters noninvasively. Here, we used in vivo Brillouin optical microscopy to characterize material acoustic properties at GHz frequency and measure the longitudinal elastic moduli of lenses. We obtained three-dimensional elasticity maps of the lenses in live mice, which showed biomechanical heterogeneity in the cortex and nucleus of the lens with high spatial resolution. An in vivo longitudinal study of mice over a period of 2 months revealed a marked age-related stiffening of the lens nucleus. We found remarkably good correlation (log-log linear) between the Brillouin elastic modulus and the Young's modulus measured by conventional mechanical techniques at low frequencies (similar to 1 Hz). Our results suggest that Brillouin microscopy is potentially useful for basic and animal research and clinical ophthalmology.
Publisher
CELL PRESS
Issue Date
2011-09
Language
English
Article Type
Article
Keywords

EYE LENS; ELASTIC PROPERTIES; REFRACTIVE-INDEX; LIGHT-SCATTERING; NUCLEAR CATARACT; BOVINE LENS; PRESBYOPIA; STIFFNESS; TRANSPARENCY; BIOMECHANICS

Citation

BIOPHYSICAL JOURNAL, v.101, no.6, pp.1539 - 1545

ISSN
0006-3495
DOI
10.1016/j.bpj.2011.08.008
URI
http://hdl.handle.net/10203/98060
Appears in Collection
NT-Journal Papers(저널논문)
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