Drop-on-Demand Patterning of Bacterial Cells Using Pulsed Jet Electrospraying

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In this study, the drop-on-demand patterning of bacterial cells on a raw silicon wafer was newly conducted with an electrospray pulsed jet. We produced various sized line patterns and spot patterns of bacterial cells on the silicon wafer under varying experimental conditions of frequency, flow rate, and translational speed of die electrospray system in one and two-dimensional ways. Especially, the electrospray's pulsed jet of bacterial solution produced regular patterns of around 10 pm diameter spots, each of which contained a single bacterial cell. By enabling drop-on-demand patterning and manipulation of single bacterial cells on a raw wafer in the gas phase, this new technique broadens the scope of biological experiments beyond liquid phase treatments to solid and gas-phase treatments.
Publisher
AMER CHEMICAL SOC
Issue Date
2010-03
Language
English
Article Type
Article
Keywords

ELECTROHYDRODYNAMIC ATOMIZATION; DEPOSITION; PROTEIN; NANOPARTICLES; MICROARRAYS; RESOLUTION; GOLD

Citation

ANALYTICAL CHEMISTRY, v.82, no.5, pp.2109 - 2112

ISSN
0003-2700
DOI
10.1021/ac9027966
URI
http://hdl.handle.net/10203/95587
Appears in Collection
ME-Journal Papers(저널논문)
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