Single-molecule FRET study of SNARE-mediated membrane fusion

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Membrane fusion is one of the most important cellular processes by which two initially distinct lipid bilayers merge their hydrophobic cores, resulting in one interconnected structure. Proteins, called SNARE (soluble N-ethylmaleimide-sensitive factor-attachment protein receptor), play a central role in the fusion process that is also regulated by several accessory proteins. In order to study the SNARE-mediated membrane fusion, the in vitro protein reconstitution assay involving ensemble FRET (fluorescence resonance energy transfer) has been used over a decade. In this mini-review, we describe several single-molecule-based FRET approaches that have been applied to this field to overcome the shortage of the bulk assay in terms of protein and fusion dynamics.
Publisher
PORTLAND PRESS LTD
Issue Date
2011-12
Language
English
Article Type
Review
Keywords

ALTERNATING-LASER EXCITATION; RESONANCE ENERGY-TRANSFER; VESICLE FUSION; COMPLEXIN BINDING; FLUORESCENCE; PROTEINS; SYNAPTOTAGMIN; RESOLUTION; REVEALS; DRIVEN

Citation

BIOSCIENCE REPORTS, v.31, no.6, pp.457 - 463

ISSN
0144-8463
URI
http://hdl.handle.net/10203/93706
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