Artificial transcription factors increase production of recombinant antibodies in Chinese hamster ovary cells

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dc.contributor.authorKwon, RJko
dc.contributor.authorKim, Sko
dc.contributor.authorLee, SIko
dc.contributor.authorHwang, SJko
dc.contributor.authorLee, Gyun-Minko
dc.contributor.authorKim, JSko
dc.contributor.authorSeol, Wko
dc.date.accessioned2013-03-08T08:14:47Z-
dc.date.available2013-03-08T08:14:47Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued2006-01-
dc.identifier.citationBIOTECHNOLOGY LETTERS, v.28, pp.9 - 15-
dc.identifier.issn0141-5492-
dc.identifier.urihttp://hdl.handle.net/10203/92574-
dc.description.abstractA randomized library that encodes for artificial zinc finger protein transcription factors (ZFP-TF) was constructed and screened for components that increased production of a monoclonal antibody (mAb-72) in Chinese hamster ovary (CHO) cells. One of these ZFP-TF, LK52, increased mAb-72 production similar to 10-fold at similar to 60% transduction efficiency; a mutated version of LK52, however, did not boost mAb-72 production. LK52 also increased production of other mAbs in CHO cells. These results demonstrate that ZFP-TF libraries can be used to identify components that improve antibody production in CHO cells.-
dc.languageEnglish-
dc.publisherSPRINGER-
dc.subjectBUTYRATE-INDUCED APOPTOSIS-
dc.subjectMAMMALIAN-CELLS-
dc.subjectPROTEIN-
dc.subjectCULTURE-
dc.subjectEXPRESSION-
dc.subjectINDUCTION-
dc.subjectLIBRARIES-
dc.subjectDNA-
dc.titleArtificial transcription factors increase production of recombinant antibodies in Chinese hamster ovary cells-
dc.typeArticle-
dc.identifier.wosid000234143800002-
dc.identifier.scopusid2-s2.0-29244486764-
dc.type.rimsART-
dc.citation.volume28-
dc.citation.beginningpage9-
dc.citation.endingpage15-
dc.citation.publicationnameBIOTECHNOLOGY LETTERS-
dc.identifier.doi10.1007/s10529-005-4680-7-
dc.contributor.localauthorLee, Gyun-Min-
dc.contributor.nonIdAuthorKwon, RJ-
dc.contributor.nonIdAuthorKim, S-
dc.contributor.nonIdAuthorLee, SI-
dc.contributor.nonIdAuthorHwang, SJ-
dc.contributor.nonIdAuthorKim, JS-
dc.contributor.nonIdAuthorSeol, W-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorartificial transcription factors-
dc.subject.keywordAuthorChinese hamster ovary cells-
dc.subject.keywordAuthorrecombinant antibody production-
dc.subject.keywordAuthorzinc finger protein-
dc.subject.keywordPlusBUTYRATE-INDUCED APOPTOSIS-
dc.subject.keywordPlusMAMMALIAN-CELLS-
dc.subject.keywordPlusPROTEIN-
dc.subject.keywordPlusCULTURE-
dc.subject.keywordPlusEXPRESSION-
dc.subject.keywordPlusINDUCTION-
dc.subject.keywordPlusLIBRARIES-
dc.subject.keywordPlusDNA-
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