Shank2 associates with and regulates Na+/H+ exchanger 3

Cited 44 time in webofscience Cited 0 time in scopus
  • Hit : 221
  • Download : 0
Na+/H+ exchanger 3 (NHE3) plays a pivotal role in transepithelial Na+ and HCO3(+) absorption across a wide range of epithelia in the digestive and renal-genitourinary systems. Accumulating evidence suggests that PDZ-based adaptor proteins play an important role in regulating the trafficking and activity of NHE3. A search for NHE3-binding modular proteins using yeast two-hybrid assays led us to the PDZ-based adaptor Shank2. The interaction between Shank2 and NHE3 was further confirmed by immunoprecipitation and surface plasmon resonance studies. When expressed in PS120/NHE3 cells, Shank2 increased the membrane expression and basal activity of NHE3 and attenuated the cAMP-dependent inhibition of NHE3 activity. Furthermore, knock-down of native Shank2 expression in Caco-2 epithelial cells by RNA interference decreased NHE3 protein expression as well as activity but amplified the inhibitory effect of cAMP on NHE3. These results indicate that Shank2 is a novel NHE3 interacting protein that is involved in the fine regulation of transepithelial salt and water transport through affecting NHE3 expression and activity.
Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Issue Date
2006-01
Language
English
Article Type
Article
Keywords

TRANSMEMBRANE CONDUCTANCE REGULATOR; PROTEIN-KINASE-A; RENAL PROXIMAL TUBULE; PDZ DOMAIN PROTEINS; APICAL MEMBRANE; POSTSYNAPTIC DENSITY; EPITHELIAL-CELLS; PANCREATIC-DUCT; HCO3-SALVAGE; H+ EXCHANGER

Citation

JOURNAL OF BIOLOGICAL CHEMISTRY, v.281, no.3, pp.1461 - 1469

ISSN
0021-9258
DOI
10.1074/jbc.M509786200
URI
http://hdl.handle.net/10203/90585
Appears in Collection
BS-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 44 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0