Flexible depth-of-field imaging system using a spatial light modulator

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A major problem of optical microscopes is their small depth-of-field (DOF), which hinders automation of micro object manipulation using visual feedback. Wavefront coding, a well-known method for extending DOF, is not suitable for direct application to micro object manipulation systems based on visual feedback owing to its expensive computational cost and due to a trade-off between the DOF and the image resolution properties. To solve such inherent problems, a flexible DOF imaging system using a spatial light modulator in the pupil plane is proposed. Especially, the trade-off relationship is quantitatively analyzed by experiments. Experimental results show that, for low criterion resolution, the DOF increases as the strength of the mask increases, while such a trend was not found for high criterion resolution. With high criterion resolution, the DOF decreases as the mask strength increases when high-resolution images are required. The results obtained can be used effectively to find the optimum mask strength given the desired image resolution. (C) 2007 Optical Society of America.
Publisher
Optical Soc Amer
Issue Date
2007-12
Language
English
Article Type
Article
Keywords

HIGH FOCAL DEPTH; EXTENDED DEPTH

Citation

APPLIED OPTICS, v.46, no.36, pp.8591 - 8599

ISSN
0003-6935
DOI
10.1364/AO.46.008591
URI
http://hdl.handle.net/10203/89810
Appears in Collection
ME-Journal Papers(저널논문)
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