Intracellular delivery of poly(ethylene glycol) conjugated antisense oligonucleotide using cationic lipids by formation of self-assembled polyelectrolyte complex micelles

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dc.contributor.authorJeong, Ji Hoonko
dc.contributor.authorKim, Sun Hwako
dc.contributor.authorKim, Sung Wanko
dc.contributor.authorPark, Tae Gwanko
dc.date.accessioned2013-03-07T02:55:36Z-
dc.date.available2013-03-07T02:55:36Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued2006-
dc.identifier.citationJOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, v.6, no.9-10, pp.2790 - 2795-
dc.identifier.issn1533-4880-
dc.identifier.urihttp://hdl.handle.net/10203/89235-
dc.description.abstractA polyelectrolyte complex (PEC) micelle-based antisense oligodeoxynucleotide (ODN) delivery system was designed to overcome intrinsic limitations of cationic lipid-mediated gene transfer. Cationic lipid (Lipofectamine(TM), LF) and ODN conjugated poly(ethylene glycol) (PEG) were ionically complexed to form self-assembled spherical PEC micelles. They have a distinctive structural feature: a charge-neutralized core surrounded by highly flexible PEG corona. The PEC micelles could be visualized as a nano-sized sphere by atomic force microscopy (AFM). The DNA/LF PEC micelles exhibited far improved transfection efficiency compared to those of conventional lipoplex formulations (ODN/LF) in the presence of serum. They showed enhanced cellular uptake followed by rapid nuclear localization of ODN in human epithelial carcinoma (KB) cells. In addition, anti-proliferative activity of c-raf gene-directed antisense ODN was almost fully maintained in KB cells in the presence of serum.-
dc.languageEnglish-
dc.publisherAmer Scientific Publishers-
dc.subjectGENE DELIVERY-
dc.subjectPHYSICOCHEMICAL PROPERTIES-
dc.subjectFUSOGENIC PEPTIDE-
dc.subjectINHIBITION-
dc.subjectLIPOSOMES-
dc.subjectSYSTEM-
dc.subjectCELLS-
dc.subjectOLIGODEOXYNUCLEOTIDES-
dc.subjectEXPRESSION-
dc.subjectSERUM-
dc.titleIntracellular delivery of poly(ethylene glycol) conjugated antisense oligonucleotide using cationic lipids by formation of self-assembled polyelectrolyte complex micelles-
dc.typeArticle-
dc.identifier.wosid000240865900017-
dc.identifier.scopusid2-s2.0-33750074452-
dc.type.rimsART-
dc.citation.volume6-
dc.citation.issue9-10-
dc.citation.beginningpage2790-
dc.citation.endingpage2795-
dc.citation.publicationnameJOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY-
dc.contributor.localauthorPark, Tae Gwan-
dc.contributor.nonIdAuthorJeong, Ji Hoon-
dc.contributor.nonIdAuthorKim, Sun Hwa-
dc.contributor.nonIdAuthorKim, Sung Wan-
dc.type.journalArticleArticle-
dc.subject.keywordAuthoroligonucleotide-
dc.subject.keywordAuthorPEG-
dc.subject.keywordAuthorpolyelectrolyte complex micelles-
dc.subject.keywordAuthornon-viral gene delivery-
dc.subject.keywordPlusGENE DELIVERY-
dc.subject.keywordPlusPHYSICOCHEMICAL PROPERTIES-
dc.subject.keywordPlusFUSOGENIC PEPTIDE-
dc.subject.keywordPlusINHIBITION-
dc.subject.keywordPlusLIPOSOMES-
dc.subject.keywordPlusSYSTEM-
dc.subject.keywordPlusCELLS-
dc.subject.keywordPlusOLIGODEOXYNUCLEOTIDES-
dc.subject.keywordPlusEXPRESSION-
dc.subject.keywordPlusSERUM-
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