Type D retrovirus gag polyprotein interacts with the cytosolic chaperonin TRiC

Cited 42 time in webofscience Cited 0 time in scopus
  • Hit : 238
  • Download : 0
The carboxy terminus-encoding portion of the gag gene of Mason-Pfizer monkey virus (M-PMV), the prototype immunosuppressive primate type D retrovirus, encodes a 36-amino-acid, proline-rich protein domain that, in the mature virion, becomes the p4 capsid protein. The p4 domain has no known role in M-PMV replication. We found that two mutants,vith premature termination codons that remove half or all of the p4 domain produced lower levels of stable Gag protein and of self-assembled capsids. Interestingly, yeast two-hybrid screening revealed that p4 specifically interacted with TCP-1 gamma, a subunit of the chaperonin TRiC (TCP-I ring complex). TRiC is a cytosolic chaperonin that is known to be involved in both folding and subunit assembly of a variety of cellular proteins. TCP-1 gamma also associated with high specificity with the M-PMV pp24/16-p12 domain and human immunodeficiency virus p6. Moreover, in cells, Gag polyprotein associated with the TRiC chaperonin complex and this association depended on ATP hydrolysis. In the p4 truncation mutants, the Gag-TRiC association was significantly reduced. These results strongly suggest that cytosolic chaperonin TRiC is involved in Gag folding and/or capsid assembly. We propose that TRiC associates transiently with nascent M-PMV Gag molecules to assist in their folding. Consequently, properly folded Gag molecules carry out the intermolecular interactions involved in self-assembly of the immature capsid.
Publisher
AMER SOC MICROBIOLOGY
Issue Date
2001-03
Language
English
Article Type
Article
Keywords

PFIZER MONKEY VIRUS; T-COMPLEX POLYPEPTIDE-1; ROUS-SARCOMA VIRUS; MOLECULAR CHAPERONES; EUKARYOTIC CYTOSOL; MATRIX PROTEIN; IN-VIVO; DNA-BINDING; BETA-ACTIN; PARTICLE

Citation

JOURNAL OF VIROLOGY, v.75, no.6, pp.2526 - 2534

ISSN
0022-538X
DOI
10.1128/JVI.75.6.2526-2534.2001
URI
http://hdl.handle.net/10203/79678
Appears in Collection
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 42 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0