DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, M | ko |
dc.contributor.author | Yoo, Ook-Joon | ko |
dc.contributor.author | Choe, SY | ko |
dc.date.accessioned | 2013-03-03T06:48:41Z | - |
dc.date.available | 2013-03-03T06:48:41Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1997-09 | - |
dc.identifier.citation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.240, no.3, pp.618 - 622 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | http://hdl.handle.net/10203/77675 | - |
dc.description.abstract | We have produced the putative extracellular domain (ECD) of the ATP-gated ion channel, P2X(2), in a bacterial expression system. The hexahistidine-tagged protein was purified by immobilized metal affinity chromatography and refolded by sulfitolysis and dialysis. We demonstrate that P2X(2)-ECD forms a stable tetramer in solution by gel filtration chromatography, dynamic light scattering and analytical sedimentation centrifugation. [alpha-P-32]ATP has been covalently cross-linked by UV irradiation to the P2X(2)-ECD and this binding is specific and competable by antagonists suramin and cibacron blue. These results indicate that the binding affinity among P2X(2)-ECD subunits is appreciably stronger than 3.4 mu M (0.1 mg/ml), implying that the extracellular domain of P2X(2) is primarly responsible for tetramerization of whole P2X(2) and thus probably plays a role in determining home-and heteromerization specificity of P2X channel subunits. (C) 1997 Academic Press. | - |
dc.language | English | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.subject | FUNCTIONAL EXPRESSION | - |
dc.subject | POTASSIUM CHANNEL | - |
dc.subject | SENSORY NEURONS | - |
dc.subject | P-2X RECEPTOR | - |
dc.subject | PURINOCEPTORS | - |
dc.subject | CLONING | - |
dc.subject | SUBUNITS | - |
dc.subject | BRAIN | - |
dc.title | Molecular assembly of the extracellular domain of P2X(2) an ATP-gated ion channel | - |
dc.type | Article | - |
dc.identifier.wosid | A1997YK69600019 | - |
dc.identifier.scopusid | 2-s2.0-0031587406 | - |
dc.type.rims | ART | - |
dc.citation.volume | 240 | - |
dc.citation.issue | 3 | - |
dc.citation.beginningpage | 618 | - |
dc.citation.endingpage | 622 | - |
dc.citation.publicationname | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.contributor.localauthor | Yoo, Ook-Joon | - |
dc.contributor.nonIdAuthor | Kim, M | - |
dc.contributor.nonIdAuthor | Choe, SY | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | FUNCTIONAL EXPRESSION | - |
dc.subject.keywordPlus | POTASSIUM CHANNEL | - |
dc.subject.keywordPlus | SENSORY NEURONS | - |
dc.subject.keywordPlus | P-2X RECEPTOR | - |
dc.subject.keywordPlus | PURINOCEPTORS | - |
dc.subject.keywordPlus | CLONING | - |
dc.subject.keywordPlus | SUBUNITS | - |
dc.subject.keywordPlus | BRAIN | - |
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