Biochemical and immunocytochemical characterization of GRIP, a putative AMPA receptor anchoring protein, in rat brain

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The mechanisms by which glutamate receptors are concentrated in brain excitatory synapses are believed to involve interactions between receptor subunits and postsynaptic anchoring or scaffolding proteins. Recently GRIP, a protein containing seven PDZ domains, was identified as an AMPA receptor binding protein and implicated in the synaptic targeting of AMPA receptors. Here we show that GRIP mRNA is also expressed in some tissues outside of the brain, including testis and kidney. Specific antibodies were raised to study GRIP protein. On Western blots, GRIP protein appears as a heterogeneous band(similar to 130 kilodaltons) which is expressed in widespread brain regions and throughout postnatal development. Biochemical studies reveal that GRIP is largely membrane associated and enriched in the postsynaptic density (PSD), though not as highly concentrated in the PSD as is PSD-95. By immunohistochemistry, GRIP is distributed in a somatodendritic pattern in neurons of adult rat brain, with especially prominent expression in a subset of interneurons. (C) 1998 Elsevier Science Ltd. All rights reserved.
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Issue Date
1998-06
Language
English
Article Type
Article; Proceedings Paper
Keywords

SELECTIVE GLUTAMATE RECEPTORS; TUMOR-SUPPRESSOR PROTEIN; POSTSYNAPTIC DENSITY; GUANYLATE KINASES; SUBUNIT COMPOSITION; CLUSTERING ACTIVITY; CA2+ PERMEABILITY; PSD-95 FAMILY; FASCICLIN-II; K+ CHANNELS

Citation

NEUROPHARMACOLOGY, v.37, no.10-11, pp.1335 - 1344

ISSN
0028-3908
DOI
10.1016/S0028-3908(98)00120-8
URI
http://hdl.handle.net/10203/77626
Appears in Collection
BS-Journal Papers(저널논문)
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