DC Field | Value | Language |
---|---|---|
dc.contributor.author | KIM, JK | ko |
dc.contributor.author | PATEL, D | ko |
dc.contributor.author | Choi, Byong-Seok | ko |
dc.date.accessioned | 2013-03-02T20:41:43Z | - |
dc.date.available | 2013-03-02T20:41:43Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1995-07 | - |
dc.identifier.citation | PHOTOCHEMISTRY AND PHOTOBIOLOGY, v.62, no.1, pp.44 - 50 | - |
dc.identifier.issn | 0031-8655 | - |
dc.identifier.uri | http://hdl.handle.net/10203/75431 | - |
dc.description.abstract | The relative biological importance of cis-syn cyclobutane dimer and pyrimidine(6-4)pyrimidone photoadduct ([6-4] photoadduct) appears to be dependent on the biological species, dipyrimidine sites and the local conformational variation induced at the damaged sites. The single-stranded deoxynucleotide 10-mers containing the site-specific (6-4) adduct or cis-syn cyclobutane dimer of thymidylyl (3'-->5')-thymidine were generated by direct photolysis of d(CGCAYTTACGC) with UVC (220-260 nm) irradiation or UVB (260-320 nm) photosensitization. Three-dimensional structures of the duplex cis-syn and (6-4) decamers of d(CGCATTACGC) d(GCGTAATGCG) were determined by NMR spectroscopy and the relaxation matrix refinement method. The NMR data and structural calculations establish that Watson-Crick base pairing is still intact at the cia-syn dimer site while the hydrogen bonding is absent at the 3'-side of the (6-4) lesion where the T --> C transition mutation is predominantly targeted. Overall conformation of the duplex cia-syn decamer was B-DNA and produced a 9 degrees bending in the DNA helix, but a distinctive base orientation of the (6-4) lesion provided a structural basis leading to 44 degrees helical bending. The observed local structure and conformational rigidity at the (6-4) adduct of the thymidylyl(3'-5')-thymidine (T-T [6-4]) lesion site suggest the potential absence of hydrogen bonding at the 3' sides of the (6-4) lesion with a substituted nucleotide during replication under SOS conditions. Contrasting structural distortions induced by the T-T (6-4) adduct with respect to the T-T cis-syn cyclobutane pyrimidine photodimer may explain the large differences in mutation spectrum and repair activities between them. | - |
dc.language | English | - |
dc.publisher | AMER SOC PHOTOBIOLOGY | - |
dc.subject | SINGLE-STRANDED VECTOR | - |
dc.subject | THYMINE DIMERS | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | NUCLEIC-ACIDS | - |
dc.subject | TRANS-SYN | - |
dc.subject | PHOTODIMERS | - |
dc.subject | PHOTOPRODUCTS | - |
dc.subject | D(GCGTTGCG).D(CGCAACGC) | - |
dc.subject | PURIFICATION | - |
dc.subject | IRRADIATION | - |
dc.title | CONTRASTING STRUCTURAL IMPACTS INDUCED BY CIS-SYN CYCLOBUTANE DIMER AND (6-4)-ADDUCT IN DNA DUPLEX DECAMERS - IMPLICATION LN MUTAGENESIS AND REPAIR ACTIVITY | - |
dc.type | Article | - |
dc.identifier.wosid | A1995RJ71000006 | - |
dc.identifier.scopusid | 2-s2.0-0029338999 | - |
dc.type.rims | ART | - |
dc.citation.volume | 62 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 44 | - |
dc.citation.endingpage | 50 | - |
dc.citation.publicationname | PHOTOCHEMISTRY AND PHOTOBIOLOGY | - |
dc.contributor.localauthor | Choi, Byong-Seok | - |
dc.contributor.nonIdAuthor | KIM, JK | - |
dc.contributor.nonIdAuthor | PATEL, D | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | SINGLE-STRANDED VECTOR | - |
dc.subject.keywordPlus | THYMINE DIMERS | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | NUCLEIC-ACIDS | - |
dc.subject.keywordPlus | TRANS-SYN | - |
dc.subject.keywordPlus | PHOTODIMERS | - |
dc.subject.keywordPlus | PHOTOPRODUCTS | - |
dc.subject.keywordPlus | D(GCGTTGCG).D(CGCAACGC) | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | IRRADIATION | - |
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