THE SOLUTION STRUCTURE OF DNA DUPLEX-DECAMER CONTAINING THE (6-4) PHOTOPRODUCT OF THYMIDYLYL(3-]5)THYMIDINE BY NMR AND RELAXATION MATRIX REFINEMENT

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The DNA duplex-decamer containing a site-specific (6-4) photoadduct of thymidylyl(3'-->5')thymidine was generated by direct photolytic ultraviolet C irradiation of d(CGCATTACGC). The three-dimensional structure of the duplex (6-4) decamer, d(CGCAT[6-4]TACGC) . d(GCGTAATGCG), has been determined by two-dimensional NMR spectroscopy and a relaxation matrix refinement method. NMR data and structural calculations established that the formation of the (6-4) adduct in the B-DNA duplex retains Watson-Crick-type hydrogen bonding throughout the duplex except at the 3'-side of the (6-4) lesion where the T-->C transition mutation is predominantly targeted [LeClerc, J. E., Borden, A. and Lawrence, C. W. (1991) Proc. Natl Acad. Sci. USA 88, 9685-9689], but leads to a 44 degrees bending in the overall DNA helix. Perpendicular base orientation of the (6-4) lesion provides a structural basis where potential hydrogen bonding at the 3' sides of the (6-4) adduct with substituted nucleotide is improbable during replication under stringent conditions.
Publisher
SPRINGER VERLAG
Issue Date
1995-03
Language
English
Article Type
Article
Keywords

CIS-SYN; ESCHERICHIA-COLI; THYMINE DIMERS; CYCLOBUTANE; H-1-NMR; REPAIR; CELLS; GENE

Citation

EUROPEAN JOURNAL OF BIOCHEMISTRY, v.228, no.3, pp.849 - 854

ISSN
0014-2956
URI
http://hdl.handle.net/10203/75430
Appears in Collection
CH-Journal Papers(저널논문)
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