DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, JH | ko |
dc.contributor.author | Hwang, GS | ko |
dc.contributor.author | Choi, Byong-Seok | ko |
dc.date.accessioned | 2013-03-02T20:33:33Z | - |
dc.date.available | 2013-03-02T20:33:33Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1999-06 | - |
dc.identifier.citation | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.96, no.12, pp.6632 - 6636 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | http://hdl.handle.net/10203/75404 | - |
dc.description.abstract | The pyrimidine(6-4)pyrimidone photoproduct [(6-4) adduct] is one of the major photoproducts induced by UV irradiation of DNA and occurs at TpT sites. The (6-4) adduct is highly mutagenic and leads most often to a 3' T --> C transition with 85% replicating error frequency [LeClerc, J E,, Borden, A. & Lawrence, C, W, (1991) Proc. Natl, Acad, Sci, USA 88, 9685-9689]. To determine the origin of the specific 3' T --> C transition of the (6-4) adduct, we have used experimental NMR restraints and molecular dynamics to determine the solution structure of a (6-4)-lesion DNA decamer duplex that contains a mismatched base pair between the 3' T residue and an opposed G residue. Normal Watson-Crick-type hydrogen bonding is retained at the 5' T of the lesion site. The O2 carbonyl of the 3' T residue forms hydrogen bonds with the imino and amino protons of the opposed C residue. This potential hydrogen bonding stabilizes the overall helix and restores the highly distorted conformation of the (6-4) adduct to the typical B-form-like DNA structure. This structural feature can explain the marked preference for the insertion of an A residue opposite the 5' T and a G residue opposite the 3' T of the (6-4) lesion during trans-lesion synthesis. Thus these insertions yield the predominant 3' T --> C transition. | - |
dc.language | English | - |
dc.publisher | NATL ACAD SCIENCES | - |
dc.subject | RELAXATION MATRIX REFINEMENT | - |
dc.subject | THYMINE CYCLOBUTANE DIMER | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | CIS-SYN | - |
dc.subject | DAMAGE RECOGNITION | - |
dc.subject | EXCISION-REPAIR | - |
dc.subject | MAMMALIAN-CELLS | - |
dc.subject | DECAMER DUPLEX | - |
dc.subject | SKIN-CANCER | - |
dc.subject | THYMIDYLYL(3&apos | - |
dc.subject | -> | - |
dc.subject | 5&apos | - |
dc.subject | )THYMIDINE | - |
dc.title | Solution structure of a DNA decanter duplex containing the stable 3 T center dot G base pair of the pyrimidine(6-4)pyrimidone photoproduct [(6-4) adduct]: Implications for the highly specific 3 T -> C transition of the (6-4) adduct | - |
dc.type | Article | - |
dc.identifier.wosid | 000080842200012 | - |
dc.type.rims | ART | - |
dc.citation.volume | 96 | - |
dc.citation.issue | 12 | - |
dc.citation.beginningpage | 6632 | - |
dc.citation.endingpage | 6636 | - |
dc.citation.publicationname | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | - |
dc.contributor.localauthor | Choi, Byong-Seok | - |
dc.contributor.nonIdAuthor | Lee, JH | - |
dc.contributor.nonIdAuthor | Hwang, GS | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | RELAXATION MATRIX REFINEMENT | - |
dc.subject.keywordPlus | THYMINE CYCLOBUTANE DIMER | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | CIS-SYN | - |
dc.subject.keywordPlus | DAMAGE RECOGNITION | - |
dc.subject.keywordPlus | EXCISION-REPAIR | - |
dc.subject.keywordPlus | MAMMALIAN-CELLS | - |
dc.subject.keywordPlus | DECAMER DUPLEX | - |
dc.subject.keywordPlus | SKIN-CANCER | - |
dc.subject.keywordPlus | THYMIDYLYL(3&apos | - |
dc.subject.keywordPlus | -> | - |
dc.subject.keywordPlus | 5&apos | - |
dc.subject.keywordPlus | )THYMIDINE | - |
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