5 processing of RNA I in an Escherichia coli strain carrying the rnpA49 mutation

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RNA I, a negative controller of ColE1-type plasmid replication, is metabolized by several RNases in Escherichia coil. Two small derivatives of RNA I are accumulated at nonpermissive temperatures in an E. coil strain carrying the mpA49 mutation, a thermosensitive mutation in the mpA gene encoding the protein component of RNase P. A primer extension analysis was carried out to compare 5' processing of RNA I in the E, coli mpA49 cells at both permissive and nonpermissive temperatures. Derivatives of RNA I having different 5' ends were observed in the cells grown at permissive and nonpermissive temperatures. Some of the derivatives may be generated by the cleavage of RNase P.
Publisher
BIOCHEMICAL SOC REPUBLIC KOREA
Issue Date
1997-03
Language
English
Article Type
Article
Keywords

RIBONUCLEASE-P; PLASMID REPLICATION; ANTISENSE RNA; MUTANTS; ENZYME; SUBUNIT; CLONING; CLEAVES

Citation

JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, v.30, no.2, pp.162 - 165

ISSN
1225-8687
URI
http://hdl.handle.net/10203/74240
Appears in Collection
CH-Journal Papers(저널논문)
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