The efficient enzymatic synthesis of N-acetyllactosamine in an organic co-solvent

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In the presence of beta-galactosidase from Bifidobacterium bifidum, N-acetyllactosamine was synthesized in significantly enhanced yield compared with earlier routes. Different proportions of the (1-->4)- and (1-->6)-linked forms were obtained depending on the choice of enzyme and reaction conditions, viz. the nature of added organic co-solvent (20-80% of 2-ethoxy ethyl ether, trimethyl phosphate, or acetone). The beta-(1 --> 4)-linked disaccharide was the major product and the beta-(1-->6)-linked disaccharide was the minor product. With beta-galactosidases from P. multicolor, A. oryzae, B. longum the beta-(1 --> 6) linkage was exclusively synthesized. Procedures for optimising the yield of N-acetyllactosamine are discussed. An immobilized enzyme on a nylon powder column was used for the efficient recycling of enzyme and synthesizing the disaccharide. (C) 2000 Elsevier Science Ltd. All rights reserved.
Publisher
ELSEVIER SCI LTD
Issue Date
2000-08
Language
English
Article Type
Article
Keywords

BETA-GALACTOSIDASES; OLIGOSACCHARIDES; DISACCHARIDES; GLYCOSIDASES; DERIVATIVES; ORIGINS

Citation

CARBOHYDRATE RESEARCH, v.327, no.4, pp.377 - 383

ISSN
0008-6215
DOI
10.1016/S0008-6215(00)00063-X
URI
http://hdl.handle.net/10203/68174
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