DYNAMICS OF ATRIAL NATRIURETIC FACTOR-GUANYLATE CYCLASE RECEPTORS AND RECEPTOR-LIGAND COMPLEXES IN CULTURED GLOMERULAR MESANGIAL AND RENOMEDULLARY INTERSTITIAL-CELLS

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The dynamics of the guanylate cyclase receptor of atrial natriuretic factor (GC(A)-ANF receptor) were investigated in cultured glomerular mesangial and renomedullary interstitial cells from the rat. In these cells, the GC(A)-ANF receptor did not mediate internalization and lysosomal hydrolysis of I-125-ANF1-28 and did not undergo ligand-induced endocytosis. Glomerular mesangial cells were able, however, to mediate internalization and lysosomal hydrolysis of I-125-ANF1-28 via clearance ANF (C-ANF) receptors and to promote rapid receptor-mediated internalization and lysosomal hydrolysis of I-125-(Sar1) angiotensin II. Radioligand specifically bound to surface GC(A)-ANF receptors was rapidly dissociated at 37-degrees-C (k(off) > 0.8 min-1), with a Q10(30-37-degrees-C) > 6. The dissociation was markedly slower at subphysiological temperatures (Q10(4-30-degrees-C), 2-3) or in the presence of 0.5 mM amiloride. The results demonstrate that the GC(A)-ANF receptor, contrary to C-ANF receptors and most other polypeptide hormone receptors, is a membrane resident protein that does not mediate internalization and lysosomal hydrolysis of ligand. The termination of the interaction of ANF with GC(A)-ANF receptors results from a physiological process that leads to rapid dissociation of receptor-ligand complexes. The unique dynamics of GC(A)-ANF receptor-ligand complexes are likely to contribute importantly to stimulus-response homeostasis of ANF.
Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Issue Date
1992-06
Language
English
Article Type
Article
Keywords

PEPTIDE CLEARANCE RECEPTOR; ADRENAL ZONA GLOMERULOSA; CYCLIC-GMP ACCUMULATION; VASCULAR SMOOTH-MUSCLE; RAT RENAL GLOMERULI; INSULIN-RECEPTORS; FUNCTIONAL EXPRESSION; CYTOPLASMIC DOMAIN; C RECEPTORS; BINDING

Citation

JOURNAL OF BIOLOGICAL CHEMISTRY, v.267, no.17, pp.11987 - 11994

ISSN
0021-9258
URI
http://hdl.handle.net/10203/67229
Appears in Collection
MSE-Journal Papers(저널논문)
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