DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Kang, Ke-Won | - |
dc.contributor.advisor | Kim, Sun-Chang | - |
dc.contributor.advisor | 강계원 | - |
dc.contributor.advisor | 김선창 | - |
dc.contributor.author | Ko, Jae-Kyoun | - |
dc.contributor.author | 고재균 | - |
dc.date.accessioned | 2011-12-12T09:01:04Z | - |
dc.date.available | 2011-12-12T09:01:04Z | - |
dc.date.issued | 1997 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=112646&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/28509 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생물과학과, 1997.2, [ vi, 47 p. ] | - |
dc.description.abstract | A cDNA encoding a guamerin has been cloned in E. coli and characterized. The guamerin is an elastase specific inhibitor with a low molecular weight of cysteine rich polypeptide isolated from Korean native leech, Hirudo nipponia. A cDNA encoding guamerin was identified by PCR amplification with degenerate primers based on its partial amino acid sequences of N-terminal and C-terminal. The deduced amino acid sequence revealed a different amino acid residue from that of guamerin. This cDNA had 71% local identity to cDNA of antistasin, factor Xa inhibitor derived from leech, Haementaria officinalis at the DNA level. The cDNA was expressed in E. coli and a 9-kDa protein was produced with inhibitory activity against neutrophil elastase as expected. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | Sequencing | - |
dc.subject | Expression | - |
dc.subject | cDNA | - |
dc.subject | 거머린 | - |
dc.subject | 클로닝 | - |
dc.subject | 염기서열분석 | - |
dc.subject | 발현 | - |
dc.subject | cDNA | - |
dc.subject | Guamerin | - |
dc.subject | Cloning | - |
dc.title | Molecular cloning, sequencing and expression of a cDNA encoding guamerin | - |
dc.title.alternative | 거머린 cDNA의 분자클로닝, 염기서열 및 발현 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 112646/325007 | - |
dc.description.department | 한국과학기술원 : 생물과학과, | - |
dc.identifier.uid | 000953018 | - |
dc.contributor.localauthor | Kang, Ke-Won | - |
dc.contributor.localauthor | Kim, Sun-Chang | - |
dc.contributor.localauthor | 강계원 | - |
dc.contributor.localauthor | 김선창 | - |
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