DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Park, Tae-Gwan | - |
dc.contributor.advisor | 박태관 | - |
dc.contributor.author | Choi, Seung-Ho | - |
dc.contributor.author | 최승호 | - |
dc.date.accessioned | 2011-12-12T08:52:28Z | - |
dc.date.available | 2011-12-12T08:52:28Z | - |
dc.date.issued | 2001 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=165535&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/27930 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생물과학과, 2001.2, [ vii, 45 p. ] | - |
dc.description.abstract | A new class of mPEG derivatives having cleavable oligo lactic acid was synthesized by ring opening polymerization of L-lactide using a terminal hydroxyl end of PEG as an initiator. The synthesized mPEG derivatives had 0.8 and 3.6 lactic acid units based on the MALDI-TOF data. A terminal end group of mPEG-OLA was further activated with p-nitrophenyl chloroformate to produce mPEG-OLA-p-nitrophenyl carbonate (PC). This mPEG-OLA-PC can conjugate to primary amine groups in proteins or drugs. In mPEG-OLA-fluorescein model system, the OLA spacer was cleaved over 40% by 12 h and most fluorescein moieties were detached from mPEG by 84 h. G-CSF was pegylated with this activated mPEG-OLA-PC. Pegylated G-CSF was consisted of native, mono-, di-, and tri-pegylated species. When incubating in pH 4 buffer at 4℃ for 2 weeks, mPEG-OLA-G-CSF liberated mPEG by cleaving OLA spacer, resulting in the reduced fraction of multi-pegylated G-CSF with concomitantly increasing the fraction of native G-CSF. In vitro NFS60 cell proliferation assay revealed that mPEG-OLA-G-CSF retained similar activity to native G-CSF. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | Lactic acid | - |
dc.subject | G-CSF | - |
dc.subject | PEGylation | - |
dc.subject | biodegradable | - |
dc.subject | 락트산 | - |
dc.subject | 생분해성 | - |
dc.subject | 단백질 개질 | - |
dc.subject | 폴리에틸렌 글리콜 접합 | - |
dc.subject | p-nitrophenyl chloroformate | - |
dc.title | Poly(ethylene glycol) conjugation of G-CSF using cleavable oligo-lactic acid spacer | - |
dc.title.alternative | 생분해성 올리고 락트산이 도입된 폴리에틸렌 글리콜의 G-CSF 접합에 관한 연구 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 165535/325007 | - |
dc.description.department | 한국과학기술원 : 생물과학과, | - |
dc.identifier.uid | 000993562 | - |
dc.contributor.localauthor | Park, Tae-Gwan | - |
dc.contributor.localauthor | 박태관 | - |
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