Poly(ethylene glycol) conjugation of G-CSF using cleavable oligo-lactic acid spacer = 생분해성 올리고 락트산이 도입된 폴리에틸렌 글리콜의 G-CSF 접합에 관한 연구

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dc.contributor.advisorPark, Tae-Gwan-
dc.contributor.advisor박태관-
dc.contributor.authorChoi, Seung-Ho-
dc.contributor.author최승호-
dc.date.accessioned2011-12-12T08:52:28Z-
dc.date.available2011-12-12T08:52:28Z-
dc.date.issued2001-
dc.identifier.urihttp://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=165535&flag=dissertation-
dc.identifier.urihttp://hdl.handle.net/10203/27930-
dc.description학위논문(석사) - 한국과학기술원 : 생물과학과, 2001.2, [ vii, 45 p. ]-
dc.description.abstractA new class of mPEG derivatives having cleavable oligo lactic acid was synthesized by ring opening polymerization of L-lactide using a terminal hydroxyl end of PEG as an initiator. The synthesized mPEG derivatives had 0.8 and 3.6 lactic acid units based on the MALDI-TOF data. A terminal end group of mPEG-OLA was further activated with p-nitrophenyl chloroformate to produce mPEG-OLA-p-nitrophenyl carbonate (PC). This mPEG-OLA-PC can conjugate to primary amine groups in proteins or drugs. In mPEG-OLA-fluorescein model system, the OLA spacer was cleaved over 40% by 12 h and most fluorescein moieties were detached from mPEG by 84 h. G-CSF was pegylated with this activated mPEG-OLA-PC. Pegylated G-CSF was consisted of native, mono-, di-, and tri-pegylated species. When incubating in pH 4 buffer at 4℃ for 2 weeks, mPEG-OLA-G-CSF liberated mPEG by cleaving OLA spacer, resulting in the reduced fraction of multi-pegylated G-CSF with concomitantly increasing the fraction of native G-CSF. In vitro NFS60 cell proliferation assay revealed that mPEG-OLA-G-CSF retained similar activity to native G-CSF.eng
dc.languageeng-
dc.publisher한국과학기술원-
dc.subjectLactic acid-
dc.subjectG-CSF-
dc.subjectPEGylation-
dc.subjectbiodegradable-
dc.subject락트산-
dc.subject생분해성-
dc.subject단백질 개질-
dc.subject폴리에틸렌 글리콜 접합-
dc.subjectp-nitrophenyl chloroformate-
dc.titlePoly(ethylene glycol) conjugation of G-CSF using cleavable oligo-lactic acid spacer = 생분해성 올리고 락트산이 도입된 폴리에틸렌 글리콜의 G-CSF 접합에 관한 연구-
dc.typeThesis(Master)-
dc.identifier.CNRN165535/325007-
dc.description.department한국과학기술원 : 생물과학과, -
dc.identifier.uid000993562-
dc.contributor.localauthorPark, Tae-Gwan-
dc.contributor.localauthor박태관-
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