Excitatory synapse formation by trans-synaptic adhesions between NGL-3 and receptor protein tyrosine phosphatases LAR, PTPdelta, and PTPsigma = NGL-3와 receptor protein tyrosine phosphatase인 LAR, PTPdelta, PTPsigma의 상호작용에 의한 흥분성 시냅스 형성에 관한 연구

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 202
  • Download : 0
Synaptic cell adhesion molecules have been involved in the control of various aspects of synaptic development. Some known synaptic adhesion molecules bind trans-synaptically and heterophilically, and regulate the synapse formation in diverse synapses. I studied that NGL-3, a PSD-95-interacting postsynaptic adhesion molecule, and LAR, a receptor protein tyrosine phosphatase, expressed in heterologous cells induce pre- and postsynaptic differentiation in contacting axons and dendrites of cocultured rat hippocampal neurons, respectively. Neuronal over expression of NGL-3 increases presynaptic contacts on dendrites of transfected neurons. Knockdown of NGL-3 reduces excitatory synapses. Competitive inhibition by soluble LAR reduces NGL-3-induced presynaptic differentiation. The first two FNIII domains of LAR are molecular determinant for interacting with NGL-3, and the other LAR family members, PTP$\delta$ and PTP$\sigma$, also can bind to NGL-3 via first two FNIII domains. These two interactions promote synapse formation in a different manner; the PTP$\sigma$-NGL-3 interaction promotes synapse formation in a bidirectional manner, whereas the PTP$\delta$-NGL-3 interaction instructs only presynaptic differentiation in a unidirectional manner. mRNAs encoding LAR family proteins display overlapping and differential expression patterns in various brain regions. These results suggest that trans-synaptic adhesion between NGL-3 and the three LAR family proteins regulates excitatory synapse formation in shared and distinct neural circuits.
Advisors
Kim, Eun-Joonresearcher김은준researcher
Description
한국과학기술원 : 생명과학과,
Publisher
한국과학기술원
Issue Date
2010
Identifier
455357/325007  / 020057034
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 생명과학과, 2010.08, [ vi, 67 p. ]

Keywords

피티피시그마; 피티피델타; 라; 엔지엘; 시냅스; synapse; PTPsigma; PTPdelta; LAR; NGL

URI
http://hdl.handle.net/10203/27706
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=455357&flag=dissertation
Appears in Collection
BS-Theses_Ph.D.(박사논문)
Files in This Item
There are no files associated with this item.

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0