Wavelength-encoded laser particles for massively multiplexed cell tagging

Cited 2 time in webofscience Cited 0 time in scopus
  • Hit : 43
  • Download : 0
Large-scale single-cell analyses have become increasingly important given the role of cellular heterogeneity in complex biological systems. However, no techniques at present enable optical imaging of uniquely tagged individual cells. Fluorescence-based approaches can distinguish only a small number of distinct cells or cell groups at a time because of spectral crosstalk between conventional fluorophores. Here we investigate large-scale cell tracking using intracellular laser particles as imaging probes that emit coherent laser light with a characteristic wavelength. Made of silica-coated semiconductor microcavities, these laser particles have single-mode emission over a broad range from 1,170 nm to 1,580 nm with sub-nanometre linewidths, enabling massive spectral multiplexing. We explore the stability and biocompatibility of these probes in vitro and their utility for wavelength-multiplexed cell tagging and imaging. We demonstrate real-time tracking of thousands of individual cells in a three-dimensional tumour model over several days, showing different behavioural phenotypes.
Publisher
NATURE PUBLISHING GROUP
Issue Date
2019-10
Language
English
Article Type
Article
Citation

NATURE PHOTONICS, v.13, no.10, pp.720 - +

ISSN
1749-4885
DOI
10.1038/s41566-019-0489-0
URI
http://hdl.handle.net/10203/267976
Appears in Collection
PH-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 2 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0