Fabrication and analysis of spheroid-hydrogel array chip using droplet contact-based transfer = 액적 접촉 기반의 이식법을 활용한 스페로이드-수화젤 어레이 칩 제작 및 분석

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In this thesis, a simple method for constructing spheroid-hydrogel array chip using droplet contact-based transfer was demonstrated. When the upper spheroid-containing drop was in contact with the lower liquid drop, the air/liquid interface disappeared at the merged surface and the spheroid settled down due to gravitational force. This method was applied to repetitive medium change and live/dead staining of spheroids cultured with the hanging drop method using a polydimethylsiloxane (PDMS)-based drop array chip (DAC) having an array of well structures and peripheral rims. To embed the spheroids into the collagen hydrogel drops, the pillar array chip (PAC) was contacted in advance with the spheroid-containing DAC. The contacted PAC had the spheroids trapped in small liquid drops whose volume were reduced to around 1/16 of the drops loaded in DAC. The spheroids were embedded in the collagen drops at once by contacting the spheroid-containing PAC and the collagen-loaded DAC. The spheroids embedded in DAC-PAC contacting method showed the slowest invasion rate, reflecting homogeneity in the concentration or distribution of collagen drops. Finally, the spheroid?collagen DAC was treated with multiple concentrations of doxorubicin and paclitaxel to demonstrate the applicability of spheroid-hydrogel array chip to anti-cancer drug assays.
Advisors
Park, Je-Kyunresearcher박제균researcher
Description
한국과학기술원 :바이오및뇌공학과,
Publisher
한국과학기술원
Issue Date
2018
Identifier
325007
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 바이오및뇌공학과, 2018.2,[vi, 51 p. :]

Keywords

3D cell culture▼aSpheroid-hydrogel array▼aHanging drop culture▼aContact-based transfer▼aSpheroid invasion assay; 3차원 세포 배양▼a스페로이드-수화젤 어레이▼a현적 배양▼a접촉 기반 이식▼a스페로이드 침투 분석

URI
http://hdl.handle.net/10203/266157
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=734183&flag=dissertation
Appears in Collection
BiS-Theses_Master(석사논문)
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