Development of multivalent based novel biomolecular probes for biochemical applications = 다중결합기반의 생체 프로브 개발 및 생화학적 응용

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Enhanced sensitivity and specificity for targeting biomolecules is a significant challenge in biomedicine. Recently, multivalent probes have been developed to improve target selectivity, but it is still in an early stage. In this dissertation, novel biomolecular probes are proposed for biochemical applications such as disease diagnostic and therapeutic. Chapter 1 provides research background of multivalent interactions. Chapter 2 describes the fabrication of protein based polymer for multivalent binding control. Rhizavidin is successfully engineered and self-assembled to produce avidin oligomer mixtures. We then purified each oligomer clearly, and characterized the properties. These new protein probes will be applied to study of multivalent interactions as protein linker. Chapter 3 represents multi-DNA probes for effective microRNA detection to enhance sensitivity and specificity. We demonstrated this three-probe complex was effective to detect microRNA with low melting temperature. This strategy was applied Surface-enhanced Raman scattering (SERS) on “hot spot” of gold surface to enhance hybridization yields. Chapter 4 represents the characterization of ferritin nanocages for active drug loading. Previously, we developed a nicked ferritin (Nicked-HF) with active drug loading. We removed unstably bounded drugs, then demonstrated doxorubicin is stably retained inside the nicked ferritin by cryoEM.
Advisors
Jung, Yongwonresearcher정용원researcher
Description
한국과학기술원 :화학과,
Publisher
한국과학기술원
Issue Date
2018
Identifier
325007
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 화학과, 2018.2,[ⅵ, 92 p. :]

Keywords

Biomolecular probes▼aMultivalent interaction; 생체 분자 프로브▼a나노 구조체▼a다중 결합

URI
http://hdl.handle.net/10203/265505
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=734465&flag=dissertation
Appears in Collection
CH-Theses_Ph.D.(박사논문)
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