Research on attachment of flagellin enhances immunostimulatory activity of hemagglutinin-ferritin nano-cage = 플라젤린 부착을 통한 헤마글루티닌-페리틴 나노케이지의 면역활성 효과의 증대에 관한 연구

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Hemagglutinin (HA) displayed on a ferritin nano-cage has been shown to be effective in generating a potent and protective immune response against a broad range of influenza infections in experimental animals. In the present study, I showed that the HA-ferritin cage produces mostly Th2 responses (class switching to IgG1), and serum IgG2a is not significantly increased. Attachment of flagellin together with HA to the exterior surface of the ferritin cage greatly enhanced not only total IgG but also Th1-type cytokine secretion and class switching to IgG2a, as a result of significant increase in Th1 responses. Moving the ferritin attachment site from the N-terminus to an internal site in flagellin had only a minor effect on immunostimulation, whereas moving it to the C-terminus completely abolished immunostimulation. I used a small immunoglobulin fragment VL12.3 as a convenient method for attaching HA and flagellin to the ferritin cage. This method can be used for rapid screening of a variety of protein cages and nano-assemblies to identify the most suitable carrier and adjuvant proteins for the target antigen.
Advisors
Lee, Jie Ohresearcher이지오researcher공동지도교수 영문명researcher공동지도교수 한글명researcher
Description
화학과,
Publisher
한국과학기술원
Issue Date
2018
Identifier
325007
Language
eng
Description

학위논문(박사) - 화학과, 2018.2,[39+iv :]

Keywords

Flagellin; Hemagglutinin-ferritin nano-cage; VL12.3; Th1 and Th2 responses; Attachment site; 플라젤린; 헤마글루티닌-페리틴 나노 케이지; Th1과 Th2 반응들; 부착 위치

URI
http://hdl.handle.net/10203/265482
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=734466&flag=dissertation
Appears in Collection
CH-Theses_Ph.D.(박사논문)
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